NDLI: The Gradient of Exciting Radiation within a Sample Affects the Relative Height of Steps in the Fast Chlorophyll a Fluorescence Rise

Content Provider	Springer Nature Link
Author	Sušila, P. Lazár, D. Ilík, P. Tomek, P. Nauš, J.
Copyright Year	2004
Abstract	Measurement of the chlorophyll (Chl) a fluorescence rise (FR) under higher exciting irradiance (EI), the O-J-I-P transient, or under lower irradiance, the O-I-P transient, is a routinely used method to access photosystem 2 function in thylakoid membranes of chloroplasts. Our measurements with a suspension of pea thylakoid membranes showed that the relative heights of the J and I steps in the FR depended not only on EI but also on the concentration and thickness of the sample. We explain this effect as a consequence of the gradient of EI within the sample. We tested this suggestion by theoretical simulations of the FR based on the model that was previously used for simulation of the FR considering in addition the gradient of EI within the sample. Our theoretical results correspond well with the experiments. The irradiance gradient effect may influence measured FR significantly and this fact should be taken into consideration in the interpretation of measured FRs.
Starting Page	161
Ending Page	172
Page Count	12
File Format	PDF
ISSN	03003604
Journal	Photosynthetica
Volume Number	42
Issue Number	2
e-ISSN	15739058
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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The Gradient of Exciting Radiation within a Sample Affects the Relative Height of Steps in the Fast Chlorophyll a Fluorescence Rise