NDLI: Drug-Loaded Polymeric Composite Skin Graft for Infection-Free Wound Healing: Fabrication, Characterization, Cell Proliferation, Migration, and Antimicrobial Activity

Content Provider	Springer Nature Link
Author	Mittal, Anupama Kumar, Neeraj
Copyright Year	2012
Abstract	A new, injectable, drug-loaded composite graft was developed to enable infection free wound healing.The graft was fabricated using gentamicin and biomimetic microparticulate scaffolds in gelatin gel and characterized for biologically relevant properties like fluid uptake, evaporative water loss (EWL), water vapor transmission rate (WVTR), Young’s modulus and degradation. It was evaluated for drug release, cytocompatibility and antimicrobial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa.Graft exhibited fluid uptake of 13.79%, EWL of 60–70% in 10 h, WVTR of 5480.31 g/m$^{2}$/d, and Young’s modulus as 2.1–10.8 kPa. It exhibited 99.36% degree of crosslinking and a dual degradation behavior wherein, the carrier gel, gelatin, degraded rapidly leaving the microparticulate scaffolds intact. Drug release studies showed a sustained release of gentamicin for 13 days sufficient to inhibit the infection at the wound site. Cytocompatibility assessment of the graft revealed that graft supported cell adhesion, proliferation and migration. The antibacterial efficacy of the graft was assessed using Kirby-Bauer method and time kill assay, wherein results indicated a quick, effective (≥5-log reduction in CFU/ml) and long lasting antimicrobial effect.These results as a whole indicate that the graft represents an effective alternative for infection-free healing of full thickness wounds. Figure Development of a drug loaded polymeric composite skin graft for infection-free wound healing: Fabrication, Water-uptake, Degradation, Cell adhesion and proliferation, Drug release, and antimicrobial activity
Starting Page	3110
Ending Page	3121
Page Count	12
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	29
Issue Number	11
e-ISSN	1573904X
Language	English
Publisher	Springer US
Publisher Date	2012-06-28
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochemistry Biomedical Engineering Pharmacy Medical Law Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fabrication of Cisplatin-Loaded Poly(lactide-co-glycolide) Composite Microspheres for Osteosarcoma Treatment

Evaluation of Wound Healing Potential of β-Chitin Hydrogel/Nano Zinc Oxide Composite Bandage

Palmitoyl Ascorbate-Loaded Polymeric Micelles: Cancer Cell Targeting and Cytotoxicity

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

A Photo-Activated Targeting Chemotherapy Using Glutathione Sensitive Camptothecin-Loaded Polymeric Micelles

Injectable SN-38-loaded Polymeric Depots for Cancer Chemotherapy of Glioblastoma Multiforme

Photocytotoxicity of mTHPC (Temoporfin) Loaded Polymeric Micelles Mediated by Lipase Catalyzed Degradation

Sonoporation of the Minicircle-VEGF$^{165}$ for Wound Healing of Diabetic Mice

Clinical Potential of a Silk Sericin-Releasing Bioactive Wound Dressing for the Treatment of Split-Thickness Skin Graft Donor Sites

Drug-Loaded Polymeric Composite Skin Graft for Infection-Free Wound Healing: Fabrication, Characterization, Cell Proliferation, Migration, and Antimicrobial Activity

Similar Documents

Fabrication of Cisplatin-Loaded Poly(lactide-co-glycolide) Composite Microspheres for Osteosarcoma Treatment

Evaluation of Wound Healing Potential of β-Chitin Hydrogel/Nano Zinc Oxide Composite Bandage

Palmitoyl Ascorbate-Loaded Polymeric Micelles: Cancer Cell Targeting and Cytotoxicity

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

A Photo-Activated Targeting Chemotherapy Using Glutathione Sensitive Camptothecin-Loaded Polymeric Micelles

Injectable SN-38-loaded Polymeric Depots for Cancer Chemotherapy of Glioblastoma Multiforme

Photocytotoxicity of mTHPC (Temoporfin) Loaded Polymeric Micelles Mediated by Lipase Catalyzed Degradation

Sonoporation of the Minicircle-VEGF$^{165}$ for Wound Healing of Diabetic Mice

Clinical Potential of a Silk Sericin-Releasing Bioactive Wound Dressing for the Treatment of Split-Thickness Skin Graft Donor Sites

Drug-Loaded Polymeric Composite Skin Graft for Infection-Free Wound Healing: Fabrication, Characterization, Cell Proliferation, Migration, and Antimicrobial Activity