NDLI: Modulation of Tumor Necrosis Factor-mediated Cell Death by Fullerenes

Content Provider	Springer Nature Link
Author	Harhaji, Ljubica Isakovic, Aleksandra Vucicevic, Ljubica Janjetovic, Kristina Misirkic, Maja Markovic, Zoran Todorovic Markovic, Biljana Nikolic, Nadezda Vranjes Djuric, Sanja Nikolic, Zoran Trajkovic, Vladimir
Copyright Year	2007
Abstract	The fullerene (C$_{60}$/C$_{70}$ mixture—C$_{60/70}$) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC$_{60/70}$) and polyhydroxylated C$_{60/70}$ [C$_{60/70}$(OH)$_{ n }$] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNF).TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes.THF/nC$_{60/70}$ augmented, while C$_{60/70}$(OH)$_{ n }$ reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC$_{60/70}$ or C$_{60/70}$(OH)$_{ n }$. The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC$_{60/70}$ or C$_{60/70}$(OH)$_{ n }$ potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization.The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.
Starting Page	1365
Ending Page	1376
Page Count	12
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	25
Issue Number	6
e-ISSN	1573904X
Language	English
Publisher	Springer US
Publisher Date	2007-11-13
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	apoptosis fullerene necrosis oxidative stress tumor necrosis factor Biomedical Engineering Medical Law Biochemistry Pharmacy Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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