NDLI: Receptor-Specific Targeting with Liposomes In Vitro Based on Sterol-PEG$

Content Provider	Springer Nature Link
Author	Gantert, Markus Lewrick, Felicitas Adrian, Joanna E. Rössler, Jochen Steenpaß, Thomas Schubert, Rolf Peschka Süss, Regine
Copyright Year	2008
Abstract	The challenge in developing liposomes to be used in active drug targeting is to design a method that can be used for modifying liposomal membranes that is applicable for a number of different specific ligands. In this study, the post insertion technique was used with activated sterol-PEG$_{1300}$ anchors and was evaluated with regard to its effectiveness in active targeting in vitro. The key advantage of these anchors is that the insertion step into the liposomal membrane takes place at room temperature and is very fast.For in vitro experiments, neuroblastoma cell lines overexpressing GD2 antigen on their surface as a target structure were chosen. This allowed the use of anti-GD2 antibodies coupled to the liposomal surface for testing of specific binding. These modified liposomes were labelled with rhodamine-PE and their cellular association was analyzed by flow cytometry.It was shown that the activated sterol-PEG$_{1300}$ anchors allow specific and significant interactions of the modified liposomes with GD2 positive cells.Coupling using sterol-PEG$_{1300}$ anchors is both simple and rapid. It is reproducible and applicable for all ligands bearing amino groups. This method demonstrates the advantage of a ready-to-use system for the modification of pre-formed liposomes with different ligands.
Starting Page	529
Ending Page	538
Page Count	10
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	26
Issue Number	3
e-ISSN	1573904X
Language	English
Publisher	Springer US
Publisher Date	2008-11-18
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedical Engineering Medical Law Biochemistry Pharmacy Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Targeting of Liposomes via PSGL1 for Enhanced Tumor Accumulation

Targeting Anticancer Drugs to Tumor Vasculature Using Cationic Liposomes

Thermosensitive Micelles from PEG-Based Ether-anhydride Triblock Copolymers

Folate-PEG-CKK$_{2}$-DTPA, A Potential Carrier for Lymph-Metastasized Tumor Targeting

Drugs Targeting Tubulin Polymerization

Microfluidic Synthesis of PEG- and Folate-Conjugated Liposomes for One-Step Formation of Targeted Stealth Nanocarriers

Targeting Efficiency of Galactosylated Liposomes to Hepatocytes in Vivo: Effect of Lipid Composition

Optimization of Acoustic Liposomes for Improved In Vitro and In Vivo Stability

Targeted Delivery to Neuroblastoma of Novel siRNA-anti-GD2-liposomes Prepared by Dual Asymmetric Centrifugation and Sterol-Based Post-Insertion Method

Receptor-Specific Targeting with Liposomes In Vitro Based on Sterol-PEG$_{1300}$ Anchors

Similar Documents

Targeting of Liposomes via PSGL1 for Enhanced Tumor Accumulation

Targeting Anticancer Drugs to Tumor Vasculature Using Cationic Liposomes

Thermosensitive Micelles from PEG-Based Ether-anhydride Triblock Copolymers

Folate-PEG-CKK$_{2}$-DTPA, A Potential Carrier for Lymph-Metastasized Tumor Targeting

Drugs Targeting Tubulin Polymerization

Microfluidic Synthesis of PEG- and Folate-Conjugated Liposomes for One-Step Formation of Targeted Stealth Nanocarriers

Targeting Efficiency of Galactosylated Liposomes to Hepatocytes in Vivo: Effect of Lipid Composition

Optimization of Acoustic Liposomes for Improved In Vitro and In Vivo Stability

Targeted Delivery to Neuroblastoma of Novel siRNA-anti-GD2-liposomes Prepared by Dual Asymmetric Centrifugation and Sterol-Based Post-Insertion Method

Receptor-Specific Targeting with Liposomes In Vitro Based on Sterol-PEG$_{1300}$ Anchors