NDLI: Polymeric Gene Transfection on Insulin-Secreting Cells: Sulfonylurea Receptor-Mediation and Transfection Medium Effect

Content Provider	Springer Nature Link
Author	Kang, Han Chang Bae, You Han
Copyright Year	2006
Abstract	In vitro transfection of secreting cells is regarded as one strategy for improved cell engineering/transplantation. Insulin-secreting insulinoma cell lines or pancreatic β-cells could be genetically engineered using designed polymeric vectors which are safer than viral vectors. This study investigates the effects of the constituents in transfection media on polymeric transfection.Polyplexes conjugated with sulfonylurea (SU) were evaluated under different transfection conditions for gene transfection and their effects on cytotoxicity and insulin secretion. Several components in transfection media specifically associated with the insulin secretion pathway were amino acids, vitamins, Ca$^{2+}$ and K$^{+}$. The interactions of the polyplexes with insulin were monitored by surface charge and particle size to monitor how insulin as a protein influences transfection.For an insulin-secreting cell line (RINm5F), polyplexes in Ca$^{2+}$-containing KRH medium (Ca$^{2+}$(+)KRH) enhanced transfection and did not cause damage to biological functions. When adding amino acids, vitamins, or K$^{+}$ or depleting Ca$^{2+}$ from Ca$^{2+}$(+)KRH, poly(l-lysine)/DNA complexes showed a greater reduction in transfection than SU receptor (SUR)-targeting polyplexes (SU-polyplex). Positively charged polyplexes interacted with insulin, developing a negative surface charge, and these interactions may cause a decrease in transfection.The findings suggest that in vitro and ex vivo polymeric transfection of insulin-secreting cells can be modulated and enhanced by adjusting the transfection conditions.
Starting Page	1797
Ending Page	1808
Page Count	12
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	23
Issue Number	8
e-ISSN	1573904X
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-07-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	insulin-secreting cells polymeric gene carrier receptor-mediated transfection sulfonylurea transfection medium Biomedical Engineering Medical Law Biochemistry Pharmacy Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

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