NDLI: Bioreducible Polypeptide Containing Cell-Penetrating Sequence for Efficient Gene Delivery

Content Provider	Springer Nature Link
Author	Chen, Si Han, Kai Yang, Juan Lei, Qi Zhuo, Ren Xi Zhang, Xian Zheng
Copyright Year	2013
Abstract	To design excellent polypeptide-based gene vectors and determine the gene delivery efficiency.Polypeptides (designated as xPolyK$_{6}$, xPolyK$_{6}$-R$_{8}$1 and xPolyK$_{6}$-R$_{8}$2), comprising the DNA condensing and buffering peptide HK$_{6}$H as well as cell penetrating peptide (CPP) R$_{8}$ were obtained by the oxidative polymerization of CHK$_{6}$HC and CR$_{8}$C at different molar ratios in 4 mL phosphate-buffered saline (PBS) containing 30% (v/v) DMSO at room temperature for 96 h. The cytotoxicity of vectors was studied by MTT assay. Moreover, particle size, zeta potential and morphology along with the in vitro transfection efficiency and cellular uptake of vector/plasmid DNA (pDNA) complexes were characterized at various w/w ratios to determine their potential in gene therapy.All the vectors presented excellent ability of binding and condensing pDNA, additionally with low cytotoxicity. Simultaneously, transfection efficiency of the vectors appeared apparent dependence on the vector composition. The distinct correlation between the content of CR$_{8}$C with the transfection efficiency demonstrated the effective improvement in transfection efficacy by the oxidative polymerization. Particularly, xPolyK$_{6}$-R$_{8}$2 possessed the highest transfection efficiency at a w/w ratio of 50. Furthermore, xPolyK$_{6}$-R$_{8}$2 also presented the best cellular uptake capability demonstrated by confocal microscopy and flow cytometry.Bioreducible polypeptides incorporating with proper amount of CPP are promising as effective non-viral gene vectors in gene therapy.
Starting Page	1968
Ending Page	1978
Page Count	11
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	30
Issue Number	8
e-ISSN	1573904X
Language	English
Publisher	Springer US
Publisher Date	2013-04-19
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pharmacology/Toxicology Pharmacy Biochemistry Medical Law Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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