NDLI: Induction of UDP-Glucuronosyltransferase UGT1A1 by the Flavonoid Chrysin in Caco-2 Cells—Potential Role in Carcinogen Bioinactivation

Content Provider	Springer Nature Link
Author	Galijatovic, Alema Otake, Yoko Walle, U. Kristina Walle, Thomas
Copyright Year	2001
Abstract	Purpose. Dietary flavonoids, present in fruits, vegetables and beverages have been demonstrated to be protective in cancer. Recently, we showed that the flavonoid chrysin induced UDP-glucuronosyl- transferase (UGT) activity and expression in the human intestinal cell line Caco-2. In the present study, we determined the specific UGT isoform(s) induced and whether this induction facilitates glucuronidation and potential detoxification of the colon carcinogen 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-hydroxy-PhIP). Methods. The induction was studied by immunoblot analysis with UGT isoform-specific antibodies, by Northern blot analysis and using quercetin as an isoform-specific catalytic probe. Glucuronidation of N-hydroxy-PhIP was characterized using both recombinant UGTs and control and chrysin-treated microsomes. Results. Western blot analysis showed that pretreatment of Caco-2 cells with 25 μM chrysin induced UGT1A1 without affecting the expression of UGTs 1A6, 1A9 and 2B7. Northern blot analysis showed markedly increased expression of UGT1A1 mRNA after chrysin treatment. Similarly, glucuronidation of quercetin was greatly increased in a UGT1A1-specific way. The induction of UGT1A1 in the Caco-2 cells resulted in a 10-fold increase in the glucuronidation of N-hydroxy-PhIP. Conclusion. Dietary flavonoid-mediated induction of intestinal UGT1A1 may be important for the glucuronidation and detoxification of colon and other carcinogens as well as for the presystemic metabolism of therapeutic drugs.
Starting Page	374
Ending Page	379
Page Count	6
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	18
Issue Number	3
e-ISSN	1573904X
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2001-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pharmacology/Toxicology Pharmacy Biochemistry Medical Law Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Induction of UDP-Glucuronosyl-Transferase by the Flavonoids Chrysin and Quercetin in Caco-2 Cells

Sex Hormones Differentially Regulate Isoforms of UDP-Glucuronosyltransferase

Identification of Human UDP-Glucuronosyltransferase Responsible for the Glucuronidation of Niflumic Acid in Human Liver

Involvement of UDP-Glucuronosyltransferases in the Extensive Liver and Intestinal First-Pass Metabolism of Flavonoid Baicalein

Breast Cancer Resistance Protein-Mediated Efflux of Luteolin Glucuronides in HeLa Cells Overexpressing UDP-Glucuronosyltransferase 1A9

Construction of a Functional Transporter Analysis System Using MDR1 Knockdown Caco-2 Cells

Physicochemical Properties and Transport of Steroids Across Caco-2 Cells

CDNA Cloning of the Mouse Bilirubin/Phenol Family of UDP-Glucuronosyltransferase (mUGTbr2-like)

Transport and Metabolism of the Tea Flavonoid (–)-Epicatechin by the Human Intestinal Cell Line Caco-2

Induction of UDP-Glucuronosyltransferase UGT1A1 by the Flavonoid Chrysin in Caco-2 Cells—Potential Role in Carcinogen Bioinactivation

Similar Documents

Induction of UDP-Glucuronosyl-Transferase by the Flavonoids Chrysin and Quercetin in Caco-2 Cells

Sex Hormones Differentially Regulate Isoforms of UDP-Glucuronosyltransferase

Identification of Human UDP-Glucuronosyltransferase Responsible for the Glucuronidation of Niflumic Acid in Human Liver

Involvement of UDP-Glucuronosyltransferases in the Extensive Liver and Intestinal First-Pass Metabolism of Flavonoid Baicalein

Breast Cancer Resistance Protein-Mediated Efflux of Luteolin Glucuronides in HeLa Cells Overexpressing UDP-Glucuronosyltransferase 1A9

Construction of a Functional Transporter Analysis System Using MDR1 Knockdown Caco-2 Cells

Physicochemical Properties and Transport of Steroids Across Caco-2 Cells

CDNA Cloning of the Mouse Bilirubin/Phenol Family of UDP-Glucuronosyltransferase (mUGTbr2-like)

Transport and Metabolism of the Tea Flavonoid (–)-Epicatechin by the Human Intestinal Cell Line Caco-2

Induction of UDP-Glucuronosyltransferase UGT1A1 by the Flavonoid Chrysin in Caco-2 Cells—Potential Role in Carcinogen Bioinactivation