NDLI: Preservation of Biomarkers from Cyanobacteria Mixed with MarsLike Regolith Under Simulated Martian Atmosphere and UV Flux

Content Provider	Springer Nature Link
Author	Baqué, Mickael Verseux, Cyprien Böttger, Ute Rabbow, Elke Vera, Jean Pierre Paul Billi, Daniela
Copyright Year	2015
Abstract	The space mission EXPOSE-R2 launched on the 24th of July 2014 to the International Space Station is carrying the BIOMEX (BIOlogy and Mars EXperiment) experiment aimed at investigating the endurance of extremophiles and stability of biomolecules under space and Mars-like conditions. In order to prepare the analyses of the returned samples, ground-based simulations were carried out in Planetary and Space Simulation facilities. During the ground-based simulations, Chroococcidiopsis cells mixed with two Martian mineral analogues (phyllosilicatic and sulfatic Mars regolith simulants) were exposed to a Martian simulated atmosphere combined or not with UV irradiation corresponding to the dose received during a 1-year-exposure in low Earth orbit (or half a Martian year on Mars). Cell survival and preservation of potential biomarkers such as photosynthetic and photoprotective pigments or DNA were assessed by colony forming ability assays, confocal laser scanning microscopy, Raman spectroscopy and PCR-based assays. DNA and photoprotective pigments (carotenoids) were detectable after simulations of the space mission (570 MJ/m$^{2}$ of UV 200–400 nm irradiation and Martian simulated atmosphere), even though signals were attenuated by the treatment. The fluorescence signal from photosynthetic pigments was differently preserved after UV irradiation, depending on the thickness of the samples. UV irradiation caused a high background fluorescence of the Martian mineral analogues, as revealed by Raman spectroscopy. Further investigation will be needed to ensure unambiguous identification and operations of future Mars missions. However, a 3-month exposure to a Martian simulated atmosphere showed no significant damaging effect on the tested cyanobacterial biosignatures, pointing out the relevance of the latter for future investigations after the EXPOSE-R2 mission. Data gathered during the ground-based simulations will contribute to interpret results from space experiments and guide our search for life on Mars.
Starting Page	289
Ending Page	310
Page Count	22
File Format	PDF
ISSN	01696149
Journal	Origins of Life and Evolution of Biospheres
Volume Number	46
Issue Number	2-3
e-ISSN	15730875
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-11-03
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Astrobiology Cyanobacteria Biosignatures Martian regoliths EXPOSE-R2 Life Sciences Astrophysics and Astroparticles Earth Sciences Astronomy, Observations and Techniques Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Ecology, Evolution, Behavior and Systematics Space and Planetary Science

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