NDLI: Imipramine-Induced Changes in 5-HT$_{2}$ Receptor Sites and Inositoltrisphosphate Levels in Rat Brain

Content Provider	Springer Nature Link
Author	Subhash, M. N. Jagadeesh, S.
Copyright Year	1997
Abstract	The effect of chronic administration of Imipramine on [$^{3}$H]Spiperone binding to 5-HT$_{2}$ sites and inositoltrisphosphate (IP$_{3}$) levels in rat cerebral cortex was studied. Our data shows that treatment with imipramine (5 mg/kg body weight, intraperitoneally) for 30 days significantly down regulates 5-HT$_{2}$ receptors sites (262 ± 29 fmol/mg protein) in cerebral cortex (38%), compared to control rats (425 ± 60 fmol/mg protein., P < 0.001). However there was no significant change in the affinity of [$^{3}$H]-Spiperone binding (kd) to 5-HT$_{2}$ sites in cerebral cortex after exposure to imipramine (Kd = 0.84 ± 0.11 nM). It is also observed that imipramine treatment significantly reduces 5-HT stimulated [$^{3}$H]IP$_{3}$ formation in cerebral cortex (6,411 ± 708 dpm/mg protein), compared to the saline treated rats (12,238 ± 1,544 dpm/mg protein; P < 0.001), with concomitant decrease in Pdtlns-4–5-P$_{2}$. This study suggests that the therapeutic action of imipramine in brain might be by reducing hypersensitivity of 5-HT$_{2}$ receptors by down regulation, which leads to reduced levels of inositolphospholipids. This inturn reduces the levels of IP$_{3}$. In conclusion, imipramine acts at presynaptic site by blocking the reuptake of serotonin and at post synaptic site it downregulates 5-HT$_{2}$ sites with decreased IP$_{3}$ levels after chronic exposure.
Starting Page	1095
Ending Page	1099
Page Count	5
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	22
Issue Number	9
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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