NDLI: The Response to Postnatal Stress: Amino Acids Transporters and PKC Activity

Content Provider	Springer Nature Link
Author	Odeon, María Mercedes Salati, Adrian Emanuel Rodríguez, Carla Beatriz Scolari, Maria José Acosta, Gabriela Beatriz
Copyright Year	2010
Abstract	It is well known that animals exposed to stressful stimuli during their early life develop different neurological disorders when they become adults. In this study, we evaluated the effect of acute cold stress on γ-aminobutyric acid (GABA) and L-Serine (L-Ser) transporters in vitro, using the uptake of [$^{3}$H]-GABA and [$^{3}$H]L-Ser by synaptosomes-enriched fractions isolated from rat cerebral cortex during postnatal development. GABA and L-Ser uptake studies in vitro will be used in this investigation as a colateral evidence of changes in the expression of transporters of GABA and L-Ser. We observed that the maximum velocity (V $_{max)}$ in L-Ser and GABA uptake after stress session increased in all stages studied. In contrast, K $_{m}$ values of L-Ser uptake enhancent in almost age calculated, excluding at PD21 after cold stress during development, at the same time as K $_{m}$ (uptake affinity) values of GABA increased in just about age considered but not at PD5 compared with the control group. Finally we investigated the mechanism by which cells regulate the substrate affinity of L-Ser and GABA transporters. We demonstrated a significantly increase in total PKC activity to PD5 from PD21. Pretreatment with PKC inhibitor: staurosporine (SP) led to a restoration of control uptake in several postnatal-days suggesting a relationship between amino acids system and PKC activation. These findings suggest that a single exposure to postnatal cold stress at different periods after birth modifies both GABA and L-Ser transporters and the related increase in total PKC activity could be intracellular events that participate in neuronal plasticity by early life stress, which could be relevant to function of transporters in the adult rat brain.
Starting Page	967
Ending Page	975
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	35
Issue Number	7
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2010-03-21
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acute cold stress GABA L-Serine Uptake PKC activity Postnatal development Transporters Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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