NDLI: Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP$^{C}$) and RNA on the Cultured Rat Cortical Neurons

Please wait, while we are loading the content...

Tricarboxylic Acid Cycle Activity Measured by $^{13}$C Magnetic Resonance Spectroscopy in Rats Subjected to the Kaolin Model of Obstructed Hydrocephalus

Low dose Estrogen Prevents Neuronal Degeneration and Microglial Reactivity in an Acute Model of Spinal Cord Injury: Effect of Dosing, Route of Administration, and Therapy Delay

Mini-Ruby is Rapidly Taken up by Neurons and Astrocytes in Organotypic Brain Slices

Histamine H$_{3}$ Receptor Agonists Decrease Hypothalamic Histamine Levels and Increase Stereotypical Biting in Mice Challenged with Methamphetamine

Role of Endoplasmic Reticulum Stress in the Amygdaloid Kindling Model of Rats

Protective Effects of Exogenous Hydrogen Sulfide on Neurons of Hippocampus in a Rat Model of Brain Ischemia

Synergistic Effects of Sodium Butyrate, a Histone Deacetylase Inhibitor, on Increase of Neurogenesis Induced by Pyridoxine and Increase of Neural Proliferation in the Mouse Dentate Gyrus

Serum Glutamine Synthetase Has No Value as a Diagnostic Biomarker for Alzheimer’s Disease

Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP$^{C}$) and RNA on the Cultured Rat Cortical Neurons

The Role of Progestogens in Regulating Matrix Metalloproteinase Activity in Macrophages and Microglial Cells

Acute Restraint Stress in Zebrafish: Behavioral Parameters and Purinergic Signaling

Castration Attenuates Myelin Repair Following Lysolecithin Induced Demyelination in Rat Optic Chiasm: An Evaluation Using Visual Evoked Potential, Marker Genes Expression and Myelin Staining

The Role of Substance P in the Marginal Division of the Neostriatum in Learning and Memory is Mediated Through the Neurokinin 1 Receptor in Rats

Neurochemical Plasticity of Nitric Oxide Synthase Isoforms in Neurogenic Detrusor Overactivity after Spinal Cord Injury

Activation of Liver X Receptor Decreases BACE1 Expression and Activity by Reducing Membrane Cholesterol Levels

Oxidative Stress, Cytokine/Chemokine and Disruption of Blood–Brain Barrier in Neonate Rats After Meningitis by Streptococcus agalactiae

Decreased Expression of Lysosomal Alpha-Galactosiase A Gene in Sporadic Parkinson’s Disease

Erratum to: Fas and FasL Expression in the Spinal Cord Following Cord Hemisection in the Monkey

Neurochemical Research : Volume 36, Issue 9, September 2011

Neurochemical Research : Volume 36, Issue 8, August 2011

Neurochemical Research : Volume 36, Issue 7, July 2011

Neurochemical Research : Volume 36, Issue 6, June 2011

Neurochemical Research : Volume 36, Issue 5, May 2011

Neurochemical Research : Volume 36, Issue 4, April 2011

Neurochemical Research : Volume 36, Issue 3, March 2011

Neurochemical Research : Volume 36, Issue 2, February 2011

Neurochemical Research : Volume 36, Issue 1, January 2011

Neurochemical Research : Volume 35

Neurochemical Research : Volume 34

Neurochemical Research : Volume 33

Neurochemical Research : Volume 32

Neurochemical Research : Volume 31

Neurochemical Research : Volume 30

Neurochemical Research : Volume 29

Neurochemical Research : Volume 28

Neurochemical Research : Volume 27

Neurochemical Research : Volume 26

Neurochemical Research : Volume 25

Neurochemical Research : Volume 24

Neurochemical Research : Volume 23

Neurochemical Research : Volume 22

Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP$^{C}$) and RNA on the Cultured Rat Cortical Neurons

Content Provider	Springer Nature Link
Author	Liu, Mei Li Wen, Jian Jun Xu, Xue Fang Zhao, De Ming
Copyright Year	2011
Abstract	Prion diseases are conformational diseases, many factors are involved in altering the conformation of prion, such as RNA, DNA, pH, and copper etc. However the neurotoxic mechanism of prion diseases is not clear yet. The aim of this study is to investigate the effect of the nucleoprotein complex of RNA and recombinant ovine prion protein (OvPrP$^{C}$) on the cultured rat cortical neurons in vitro. Our previous study revealed that the nucleoprotein complex (OvPrP$^{C}$-RNA) is characterized with high β sheet conformation and proteinase K resistance. Here we found that the OvPrP$^{C}$-RNA induced marked neuronal cell death by the MTT (3-(4,5-dimethyl-thiazole -2-yl)-2,5-diphenyl –tetrazolium bromide) and TUNEL (TdT mediated biotin-dUTP nicked-end labeling) assay, and the neurotoxic effects were confirmed by testing the content of Bcl-2 Associated X protein (Bax) in the immunoprecipitation assay and Western blot assay. Compared to the control group, there is no significant difference of active Bax or total Bax after RNA alone treatment or OvPrP$^{C}$ alone treatment, but the OvPrP$^{C}$-RNA induced significant increases of active Bax level, while the contents of total Bax had no obvious changes after OvPrP$^{C}$-RNA treatment. The results suggested that OvPrP$^{C}$-RNA is neurotoxic in vitro, which added further evidence to the current understanding of mechanism of cellular injury by RNA molecules for transformation of the PrP$^{C}$ to PrP$^{Sc}$.
Starting Page	1863
Ending Page	1869
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	36
Issue Number	10
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2011-05-24
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Prion OvPrP$^{C}$-RNA Neuronal injury neurotoxicity Bax Neurology Cell Biology Neurochemistry Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Concentration-Dependent Dual Role of Thrombin in Protection of Cultured Rat Cortical Neurons

Proteomic Analysis of Primary Cultured Rat Cortical Neurons in Chemical Ischemia

The Unexposed Secrets of Prion Protein Oligomers

Protective Effects of Humanin on Okadaic Acid-Induced Neurotoxicities in Cultured Cortical Neurons

The Ketone Body, β-Hydroxybutyrate Stimulates the Autophagic Flux and Prevents Neuronal Death Induced by Glucose Deprivation in Cortical Cultured Neurons

Release of Full-Length PrPC from Cultured Neurons Following Neurotoxic Challenge

The cellular prion protein promotes neuronal regeneration after acute nasotoxic injury

Acute exposure to ethanol potentiates human immunodeficiency virus type 1 Tat-induced Ca$^{2+}$ overload and neuronal death in cultured rat cortical neurons

Potential Protection of Green Tea Polyphenols Against 1800 MHz Electromagnetic Radiation-Induced Injury on Rat Cortical Neurons

Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP$^{C}$) and RNA on the Cultured Rat Cortical Neurons

Similar Documents

Concentration-Dependent Dual Role of Thrombin in Protection of Cultured Rat Cortical Neurons

Proteomic Analysis of Primary Cultured Rat Cortical Neurons in Chemical Ischemia

The Unexposed Secrets of Prion Protein Oligomers

Protective Effects of Humanin on Okadaic Acid-Induced Neurotoxicities in Cultured Cortical Neurons

The Ketone Body, β-Hydroxybutyrate Stimulates the Autophagic Flux and Prevents Neuronal Death Induced by Glucose Deprivation in Cortical Cultured Neurons

Release of Full-Length PrPC from Cultured Neurons Following Neurotoxic Challenge

The cellular prion protein promotes neuronal regeneration after acute nasotoxic injury

Acute exposure to ethanol potentiates human immunodeficiency virus type 1 Tat-induced Ca$^{2+}$ overload and neuronal death in cultured rat cortical neurons

Potential Protection of Green Tea Polyphenols Against 1800 MHz Electromagnetic Radiation-Induced Injury on Rat Cortical Neurons

Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP$^{C}$) and RNA on the Cultured Rat Cortical Neurons