NDLI: In Vitro Effects of Arachidonic and L-Glutamic Acids on the High-Affinity Choline Transport in Rat Hippocampus

Content Provider	Springer Nature Link
Author	Krištofiková, Zdena Klaschka, Jan
Copyright Year	1997
Abstract	A second messenger role for arachidonic acid (AA) in the regulation of the high-affinity choline uptake (HACU) was suggested. It was repotted that micromolar concentrations of AA applied in vitro decreased the HACU values and increased the specific binding of [$^{3}$H]hemicholinium-3 ([$^{3}$H]HCh-3). It was published that L-glutamic acid (GA) applied in vivo produced a fall in the HACU values. In addition, GA liberates free AA. In this study, an ability of GA to influence in vitro the activity of presynaptic cholinergic nerve terminals via its effect on the release of AA is investigated in hippocampal synaptosomes of young Wistar rats. Millimolar concentrations of GA decrease both the high- and low-affinity choline uptake, the specific as well as nonspecific binding of [$^{3}$H]HCh-3 and the activity of Na$^{+}$,K$^{+}$-ATPase. Kinetic analysis (Lineweaver-Burk and Scatchard plots) reveals a change in V$_{max}$ and B$_{max}$, but not in K$_{M}$ and K$_{D}$. It appears very likely that under normal conditions GA applied in vitro is not able to change markedly the choline transport via its effect on the release of AA. Results confirm the hypothesis about an indirect inhibitory role for glutamatergic receptors on cholinergic cells.
Starting Page	67
Ending Page	73
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	22
Issue Number	1
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of K$^{+}$-Depolarization, Arachidonic Acid, Ethanol, and Aging on the High-Affinity Choline Transport in Rat Hippocampus

High-Affinity Choline Transporter

Estrogen Effects on High-Affinity Choline Uptake in Primary Cultures of Rat Basal Forebrain

Sex-dependent Actions of Amyloid Beta Peptides on Hippocampal Choline Carriers of Postnatal Rats

Simultaneous Isolation of Glial and Neuronal Fractions from Rat Brain Homogenates: Comparison of High-Affinity L-Glutamate Transport Properties

Na$^{+}$-Independent Choline Transport in Rat Retinal Capillary Endothelial Cells

Effects of Aging and Amyloid-β Peptides on Choline Acetyltransferase Activity in Rat Brain

Effects of Neonatal Hypoxia/Ischemia on Ganglioside Expression in the Rat Hippocampus

Choline Availability Modulates the Expression of TGFβ1 and Cytoskeletal Proteins in the Hippocampus of Developing Rat Brain

In Vitro Effects of Arachidonic and L-Glutamic Acids on the High-Affinity Choline Transport in Rat Hippocampus

Similar Documents

Effects of K$^{+}$-Depolarization, Arachidonic Acid, Ethanol, and Aging on the High-Affinity Choline Transport in Rat Hippocampus

High-Affinity Choline Transporter

Estrogen Effects on High-Affinity Choline Uptake in Primary Cultures of Rat Basal Forebrain

Sex-dependent Actions of Amyloid Beta Peptides on Hippocampal Choline Carriers of Postnatal Rats

Simultaneous Isolation of Glial and Neuronal Fractions from Rat Brain Homogenates: Comparison of High-Affinity L-Glutamate Transport Properties

Na$^{+}$-Independent Choline Transport in Rat Retinal Capillary Endothelial Cells

Effects of Aging and Amyloid-β Peptides on Choline Acetyltransferase Activity in Rat Brain

Effects of Neonatal Hypoxia/Ischemia on Ganglioside Expression in the Rat Hippocampus

Choline Availability Modulates the Expression of TGFβ1 and Cytoskeletal Proteins in the Hippocampus of Developing Rat Brain

In Vitro Effects of Arachidonic and L-Glutamic Acids on the High-Affinity Choline Transport in Rat Hippocampus