NDLI: Tetramethylpyrazine Analogue CXC195 Ameliorates Cerebral Ischemia–reperfusion Injury by Regulating Endothelial Nitric Oxide Synthase Phosphorylation Via PI3K/Akt Signaling

Content Provider	Springer Nature Link
Author	Yan, Shi Chen, Lin Wei, Xinbing Cheng, Li Kong, Lingjun Liu, Xinyong Zhang, Xiumei Liu, Huiqing
Copyright Year	2014
Abstract	A novel tetramethylpyrazine derivative, CXC195, has been recently shown to protect against cerebral ischemia–reperfusion (I/R) injury. However, the detailed mechanisms underlying the neuroprotection of CXC195 are still unclear. The aim of the present study was to investigate the effects of CXC195 on the phosphorylation of endothelial nitric oxide synthase (eNOS) in response to cerebral I/R and to determine whether phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway might be involved. An in vitro model of oxygen glucose deprivation (OGD) which was performed on primary cultured human aortic endothelial cells (HAECs) and an in vivo middle cerebral artery occlusion (MCAO) model that was performed on Wistar rats were used in this study. CXC195 increased nitric oxide (NO) production and the phosphorylation but not the protein level of eNOS in HAECs subjected to 1 h OGD followed by reperfusion. In addition, CXC195 increased the phosphorylation of Akt; inhibition of PI3K/Akt pathway by a specific inhibitor, wortmannin, suppressed CXC195-induced NO release in HAECs. Consistently, CXC195 treatment significantly restored the phosphorylations of eNOS and Akt in the cortical penumbra of rats subjected to 2 h MCAO followed by reperfusion. Moreover, wortmannin abolished CXC195-induced eNOS phosphorylation and neuroprotection as evidenced by a reversal of the reduction in infarct volume and neurobehavioral outcomes. In conclusion, CXC195 induced phosphorylation of eNOS by activation of PI3K/Akt signaling under pathological cerebral I/R conditions, which provided a novel explanation for the neuroprotective effect of CXC195.
Starting Page	446
Ending Page	454
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	40
Issue Number	3
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2014-11-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tetramethylpyrazine derivative CXC195 Cerebral ischemia–reperfusion Endothelial nitric oxide synthase Akt Phosphorylation PI3K Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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