NDLI: Expression of Tyrosine Hydroxylase is Negatively Regulated Via Prion Protein

Content Provider	Springer Nature Link
Author	Luz, Marcio Henrique Mello Glezer, Isaias Xavier, Andre Machado Silva, Marcelo Alberti Paiva Pi, Jessica Monteiro Volejnik Zamith, Thiago Panaro Vieira, Taynara Fernanda Antonio, Bru Brito Antunes, Hanna Karen Moreira Martins, Vilma Regina Lee, Kil Sun
Copyright Year	2016
Abstract	Cellular prion protein (PrP$^{C}$) is a glycoprotein of the plasma membrane that plays pleiotropic functions by interacting with multiple signaling complexes at the cell surface. Recently, a number of studies have reported the involvement of PrP$^{C}$ in dopamine metabolism and signaling, including its interactions with tyrosine hydroxylase (TH) and dopamine receptors. However, the outcomes reported by independent studies are still debatable. Therefore in this study, we investigated the effects of PrP$^{C}$ on the TH expression during the differentiation of N2a cells with dibutyryl-cAMP, a well-known cAMP analog that activates TH transcription. Upon differentiation, TH was induced with concomitant reduction of PrP$^{C}$ at protein level, but not at mRNA level. shRNA-mediated PrP$^{C}$ reduction increased the basal level of TH at both mRNA and protein levels without dibutyryl-cAMP treatment. This phenotype was reversed by re-expression of PrP$^{C}$. PrP$^{C}$ knockdown also potentiated the effect of dibutyryl-cAMP on TH expression. Our findings suggest that PrP$^{C}$ has suppressive effects on TH expression. As a consequence, altered PrP$^{C}$ functions may affect the regulation of dopamine metabolism and related neurological disorders.
Starting Page	1691
Ending Page	1699
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	41
Issue Number	7
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2016-03-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellular prion protein Dopamine metabolism Tyrosine hydroxylase Gene expression Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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