NDLI: Role of Astrocytes in Depolarization-Coupled Release of Glutamate in Cerebellar Cultures

Content Provider	Springer Nature Link
Author	Bak, Lasse K. Waagepetersen, Helle S. Schousboe, Arne
Copyright Year	2004
Abstract	Release of preloaded D-[$^{3}$H]aspartate in response to depolarization induced by high potassium, N-methyl-D-aspartate (NMDA), α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) or the endogenous agonist glutamate was studied using cultured glutamatergic cerebellar granule neurons, cerebellar astrocytes, and corresponding cocultures. Release from the vesicular and the cytoplasmic glutamate pools, respectively, was distinguished employing the competitive, nontransportable glutamate transport inhibitor dl-threo-β-benzyloxyaspartate (dl-TBOA). The results indicate that the release in response to AMPA (30 μM) in the presence of cyclothiazide (50 μM) to block desensitization, was of a vesicular origin. Pulses of 55 mM K$^{+}$ caused a dl-TBOA resistant efflux of preloaded D-[$^{3}$H]aspartate from astrocytes, indicating that this release was not mediated by glutamate transporters. The results furthermore support the notion of an important function of the astrocytes in the uptake of released glutamate, because dl-TBOA caused a large, apparent increase in the depolarization-coupled release of preloaded D-[$^{3}$H]aspartate in the cocultures, compared to neuronal monocultures.
Starting Page	257
Ending Page	265
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	29
Issue Number	1
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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