NDLI: Hydrogen Peroxide Induces Lysosomal Protease Alterations in PC12 Cells

Content Provider	Springer Nature Link
Author	Lee, Daniel C. Mason, Ceceile W. Goodman, Carl B. Holder, Maurice S. Kirksey, Otis W. Womble, Tracy A. Severs, Walter B. Palm, Donald E.
Copyright Year	2007
Abstract	Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H$_{2}$O$_{2}$. Furthermore, activation of CATD occurred following exposure to H$_{2}$O$_{2}$ and cysteine protease suppression, while inhibition of CATD with pepstatin A significantly improved cell viability. Additionally, significant PARP cleavage, suggestive of caspase-3-like activity, was observed following H$_{2}$O$_{2}$ exposure, while inhibition of caspase-3 significantly increased cell viability compared to H$_{2}$O$_{2}$ administration alone. Collectively, our data suggest that H$_{2}$O$_{2}$ induced cell death is regulated at least in part by caspase-3 and CATD. Furthermore, cysteine protease suppression increases CATD expression and activity. These studies provide insight for alternate pathways and potential therapeutic targets of cell death associated with oxidative stress and lysosomal protease alterations.
Starting Page	1499
Ending Page	1510
Page Count	12
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	32
Issue Number	9
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2007-04-18
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cystatin C Lysosomes Cathepsin H$_{2}$O$_{2}$ PC12 cells Neurodegeneration Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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