NDLI: Validated Genomic Approach to Study Differentially Expressed Genes in Complex Tissues

Content Provider	Springer Nature Link
Author	Wurmbach, Elisa González Maeso, Javier Yuen, Tony Ebersole, Barbara J. Mastaitis, Jason W. Mobbs, Charles V. Sealfon, Stuart C.
Copyright Year	2002
Abstract	Microarray-based genomic techniques allow the simultaneous determination of relative levels of expression of a large number of genes. Studies of the transcriptome in complex neurobiological systems are uniquely demanding due to the heterogeneous nature of these cells. Most brain regions contain a large variety of cell populations that are closely intermingled. The expression of any specific gene may be restricted to a subpopulation of cells, and changes in gene expression may occur in only a small fraction of the cells expressing that transcript. Due to this dilution effect, many genes of interest are expected to have relatively low levels of expression in tissue homogenates. Furthermore, biologically significant differences in expression may result in only small fold-changes. Therefore genomic approaches using brain dissections must be optimized to identify potentially regulated transcripts and differential expression should be confirmed using quantitative assays. We evaluated the effects of increasing tissue complexity on detection of regulated transcripts in focused microarray studies using a mouse cell line, mouse hypothalamus and mouse cortex. Regulated transcripts were confirmed by quantitative real-time PCR. As tissue complexity increased, distinguishing significantly regulated genes from background variation became increasingly more difficult. However, we found that cDNA microarray studies using regional brain dissections and appropriate numbers of replicates could identify genes showing less than 2-fold regulation and that most regulated genes identified fell within this range.
Starting Page	1027
Ending Page	1033
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	27
Issue Number	10
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2002-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cloning and Identification of Differentially Expressed Transcripts in Primary Culture of GABAergic Neurons

Oligonucleotide-based Analysis of Differentially Expressed Genes in Hippocampus of Transgenic Mice Expressing NSE-controlled APPsw

RNA Amplification in Brain Tissues

Identification of Regulatory Relationships in Parkinson's Disease

α-Synuclein is expressed in different tissues during human fetal development

Differentially Expressed Genes in Transgenic Mice Carrying Human Mutant Presenilin-2 (N141I): Correlation of Selenoprotein M with Alzheimer’s Disease

Energetics of Functional Activation in Neural Tissues

Neuregulin-1 isoforms are differentially expressed in the intact and regenerating adult rat nervous system

Target Discovery and Validation in the Post-Genomic Era

Validated Genomic Approach to Study Differentially Expressed Genes in Complex Tissues

Similar Documents

Cloning and Identification of Differentially Expressed Transcripts in Primary Culture of GABAergic Neurons

Oligonucleotide-based Analysis of Differentially Expressed Genes in Hippocampus of Transgenic Mice Expressing NSE-controlled APPsw

RNA Amplification in Brain Tissues

Identification of Regulatory Relationships in Parkinson's Disease

α-Synuclein is expressed in different tissues during human fetal development

Differentially Expressed Genes in Transgenic Mice Carrying Human Mutant Presenilin-2 (N141I): Correlation of Selenoprotein M with Alzheimer’s Disease

Energetics of Functional Activation in Neural Tissues

Neuregulin-1 isoforms are differentially expressed in the intact and regenerating adult rat nervous system

Target Discovery and Validation in the Post-Genomic Era

Validated Genomic Approach to Study Differentially Expressed Genes in Complex Tissues