NDLI: Serotonin Modulation of Low-Affinity Ouabain Binding in Rat Brain Determined by Quantitative Autoradiography

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Neurochemical Research : Volume 23, Issue 6, June 1998

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Neurochemical Research : Volume 22

Serotonin Modulation of Low-Affinity Ouabain Binding in Rat Brain Determined by Quantitative Autoradiography

Content Provider	Springer Nature Link
Author	Antonelli, Marta C. Lieste, Marcela Costa Mercado, Rosalio Hernández R, Jorge
Copyright Year	1998
Abstract	Previous results showed that Na$^{+}$/K$^{+}$-ATPase may have a functional relationship with the neurotransmitter serotonin which activates the glial sodium pump in the rat brain. Both the reaction rate (V) of Na$^{+}$/K$^{+}$-ATPase activity and [$^{3}$H]ouabain binding were significantly increased in the presence of serotonin. It is not known, however, which α isoform is involved in the Na$^{+}$/K$^{+}$-ATPase response to serotonin and its regional distribution. Quantitative autoradiography of [$^{3}$H]ouabain binding to rat brain slices was employed at different [$^{3}$H]ouabain concentrations in order to gain information on both the distribution and the possible isoform involved. The results showed that 1500 nM [$^{3}$H]ouabain binding was sensitive to serotonin 10$^{−3}$ M and significantly increased in the following brain regions: frontal cortex, areas CA1, CA2, and CA3 of the hippocampus, presubiculum, zona incerta, caudate putamen and the amygdaloid area, confirming and extending previous results. An effect of serotonin on brain but not kidney tissue at high, 1500 nM, and the lack of effect at low, 50 nM [$^{3}$H]ouabain concentrations, strongly suggests the participation of the α2 isoform in the response of the pump to the neurotransmitter. Glial cells showed stimulation of ouabain binding by serotonin at ouabain concentrations above 350 nM. The present results open interesting questions related to the brain regions involved and the K$^{+}$ handling by the glial α2 isoform of the pump.
Starting Page	939
Ending Page	944
Page Count	6
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	23
Issue Number	7
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1998-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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