NDLI: Association of Cellular Prion Protein with Gangliosides in Plasma Membrane Microdomains of Neural and Lymphocytic Cells

Content Provider	Springer Nature Link
Author	Mattei, Vincenzo Garofalo, Tina Misasi, Roberta Gizzi, Chiara Mascelli, Maria Teresa Dolo, Vincenza Pontieri, Giuseppe M. Sorice, Maurizio Pavan, Antonio
Copyright Year	2002
Abstract	In this report we demonstrated that cellular prion protein is strictly associated with gangliosides in microdomains of neural and lymphocytic cells. We preliminarily investigated the protein distribution on the plasma membrane of human neuroblastoma cells, revealing the presence of large clusters. In order to evaluate its possible role in tyrosine signaling pathway triggered by GEM, we analyzed PrP$^{c}$ presence in microdomains and its association with gangliosides, using cholera toxin as a marker of GEM in neuroblastoma cells and anti-GM3 MoAb for identification of GEM in lymphoblastoid cells. In neuroblastoma cells scanning confocal microscopical analysis revealed a consistent colocalization between PrP$^{c}$ and GM1 despite an uneven distribution of both on the cell surface, indicating the existence of PrP$^{c}$-enriched microdomains. In lymphoblastoid T cells PrP$^{c}$ molecules were mainly, but not exclusively, colocalized with GM3. In addition, PrP$^{c}$ was present in the Triton-insoluble fractions, corresponding to GEM of cell plasma membrane. Additional evidence for a specific PrP$^{c}$-GM3 interaction in these cells was derived from the results of TLC analysis, showing that prion protein was associated with GM3 in PrP$^{c}$ immunoprecipitates. The physical association of PrP$^{c}$ with ganglioside GM3 within microdomains of lymphocytic cells strongly suggests a role for PrP$^{c}$-GM3 complex as a structural component of the multimolecular signaling complex involved in T cell activation and other dynamic lymphocytic plasma membrane functions.
Starting Page	743
Ending Page	749
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	27
Issue Number	7-8
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2002-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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