NDLI: Hydrolysis of Substance P in the Presence of the Osteosarcoma Cell Line SaOS-2: Release of Free Amino Acids

Content Provider	Springer Nature Link
Author	Cavazza, Antonella Marini, Mario Roda, L. Giorgio Taranti, Umberto Valenti, Angela
Copyright Year	2011
Abstract	The possible hydrolysis of substance P (Arg–Pro–Lys–Pro–Gln–Gln–Phe–Phe–Gly–Leu–Met) in presence of the osteoblastic cell line SaOS-2 was measured by capillary electrophoresis coupled to mass detection. The results obtained indicate that a very rapid disappearance of the intact undecapeptide was associated to a slower appearance of seven of its eight component amino acids. These results can be interpreted as indicating that an extremely fast hydrolysis of substance P by endopeptidases, which released peptidic by-products, was followed by a noticeably slower secondary degradation which released free amino acids. In decreasing quantitative importance, these phenomena appear to originate by the hydrolysis of the Pro$_{4}$–Gln$_{5}$ bond, followed by C-terminal sequential degradation of the Arg$_{1}$–Pro$_{4}$ tetrapeptide; by the hydrolysis of or Phe$_{7}$–Phe$_{8}$ bond (or, possibly, of Gln$_{6}$–Phe$_{7}$) leading to release of free Phe and Gln; by hydrolysis of the Gly$_{9}$–Leu$_{10}$ bond with subsequent release of Met and Leu. Results obtained appear to be compatible with the expression by SaOS-2 cells of enzymes already known to catalyze substance P hydrolysis, together with an apparent low efficiency of aminopeptidases. Because of the activity of C-terminal fragments on NK1 receptors, the delay between primary hydrolysis of substance P and secondary hydrolysis of its peptidic fragments indicated by the data shown implies a possible persistence of substance P physiological effects even after degradation of the intact peptide.
Starting Page	2339
Ending Page	2345
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	36
Issue Number	12
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2011-08-21
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neuropeptides Osteosarcoma cell line Enzyme hydrolysis Neurochemistry Cell Biology Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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