NDLI: Moderate Dependence of ROS Formation on ΔΨm in Isolated Brain Mitochondria Supported by NADH-linked Substrates

Content Provider	Springer Nature Link
Author	Tretter, Laszlo Adam Vizi, Vera
Copyright Year	2006
Abstract	The membrane potential (ΔΨm) dependence of the generation of reactive oxygen species (ROS) in isolated guinea-pig brain mitochondria respiring on NADH-linked substrates (glutamate plus malate) was addressed. Depolarization by FCCP was without effect on H$_{2}$O$_{2}$ formation in the absence of bovine serum albumin (BSA). Addition of BSA (0.025%) to the assay medium hyperpolarized mitochondria by 6.1 ± 0.9 mV (from 169 ± 3 to 175.1 ± 2.1 mV) and increased the rate of H$_{2}$O$_{2}$ formation from 207 ± 4.5 to 312 ± 12 pmol/min/mg protein. Depolarization by FCCP (5–250 nM) in the presence of BSA decreased H$_{2}$O$_{2}$ formation but only to the level observed in the absence of BSA. Rotenone stimulated the formation of H$_{2}$O$_{2}$ both in the absence and presence of BSA. It is suggested that H$_{2}$O$_{2}$ formation in mitochondria supported by NADH-linked substrates is sensitive to changes in ΔΨm only when mitochondria are highly polarized and even then, 60% of ROS generation is independent of ΔΨm. This is in contrast to earlier reports on the highly ΔΨm sensitive ROS formation related to reverse electron flow observed in well-coupled succinate-supported mitochondria.
Starting Page	569
Ending Page	575
Page Count	7
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	32
Issue Number	4-5
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-08-24
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mitochondria Reactive oxygen species Membrane potential Amplex red Glutamate–malate Respiratory chain NADH Complex I Hydrogen peroxide Superoxide Bovine serum albumin Brain Guinea pig Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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