NDLI: Mechanisms Underlying Leakage of Calcium from the Endoplasmic Reticulum of Acinar Cells of the Submandibular Salivary Gland

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Mechanisms Underlying Leakage of Calcium from the Endoplasmic Reticulum of Acinar Cells of the Submandibular Salivary Gland

Content Provider	Springer Nature Link
Author	Kopach, O. V. Kruglikov, I. A. Kostyuk, P. G. Voitenko, N. V. Fedirko, N. V.
Copyright Year	2005
Abstract	In the resting state, the Ca$^{2+}$ concentration in agonist-sensitive intracellular stores reflects the balance between active uptake of Ca$^{2+}$, which is mediated by Ca$^{2+}$-ATPase (SERCA), and passive leakage of Ca$^{2+}$. The mechanisms underlying such a leakage in cells of the submaxillary salivary gland were not studied. In our experiments, we examined possible pathways of passive leakage of Ca$^{2+}$ from the endoplasmic reticulum (ER) of acinar cells obtained from the rat submaxillary salivary gland; direct measurements of the concentration of Ca$^{2+}$ in the ER ([Ca$^{2+}$]$_{ER}$) using a low-affinity calcium-sensitive dye, mag-fura 2/AM, were performed. The cellular membrane was permeabilized with the help of β-escin (40 μg/ml); the Ca$^{2+}$ concentration in the cytoplasm ([Ca$^{2+}$]$_{ i }$) was clamped at its level typical of the resting state (∼100 nM) using an EGTA/Ca$^{2+}$ buffer. Incubation of permeabilized acinar cells in a calcium-free intracellular milieu, as well as application of thapsigargin, resulted in complete inhibition of the uptake of Ca$^{2+}$ with the involvement of SERCA. This effect was observed 1 min after the beginning of superfusion of the cells with the corresponding solutions and was accompanied by the leakage of Ca$^{2+}$ from the ER; this is confirmed by a gradual drop in the [Ca$_{2+}$]$_{ER}$. Such a leakage of Ca$^{2+}$ remained unchanged in the presence of thapsigargin, heparin, and ruthenium red; therefore, it is not mediated by SERCA, inositol 1,4,5-trisphosphate-sensitive receptors (InsP$_{3}$R), or ryanodine receptors (RyRs). At the same time, an antibiotic, puromycin (0.1 to 1.0 mM), which disconnects polypeptides from the ER-ribosome translocon complex, caused intensification of passive leakage of Ca$^{2+}$ from the ER. This effect did not depend on the functioning of SERCA, InsP$_{3}$R, or RyR. Therefore, passive leakage of Ca$^{2+}$ from the ER in acinar cells of the submaxillary salivary gland is realized through pores of the translocon complex of the ER membrane.
Starting Page	296
Ending Page	302
Page Count	7
File Format	PDF
ISSN	00902977
Journal	Neurophysiology
Volume Number	37
Issue Number	4
e-ISSN	15739007
Language	English
Publisher	Kluwer Academic Publishers-Consultants Bureau
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	intracellular calcium concentration endoplasmic reticulum acinar cells Ca$^{2+}$/Mg$^{2+}$-ATPases InsP$_{3}$ receptor ryanodine receptor Neurosciences
Content Type	Text
Resource Type	Article
Subject	Neuroscience Physiology

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