NDLI: Quantitative analysis of gold and carbon nanoparticles in mammalian cells by flow cytometry light scattering

Content Provider	Springer Nature Link
Author	Zhou, Gang Liu, Naicheng Wang, Zhenheng Shi, Tongguo Gan, Jingjing Wang, Zhenzhen Zhang, Junfeng
Copyright Year	2017
Abstract	Nanoparticle-based applications for diagnostics and therapeutics have been extensively studied. These applications require a profound understanding of the fate of nanoparticles (NPs) in cellular environments. However, until now, few analytical methods are available and most of them rely on fluorescent properties or special elements of NPs; therefore, for NPs without observable optical properties or special elements, the existing methods are hardly applicable. In this study, we introduce a flow cytometry light scattering (FCLS)-based approach that quantifies in situ NPs accurately in mammalian cells. Continuous cells of heterogeneous human epithelial colorectal adenocarcinoma (Caco-2 cells), mouse peritoneal macrophages (MPM), and human adenocarcinomic alveolar basal epithelia (A549 cells) were cultured with NPs with certain concentrations and size. The intensity of the flow cytometric side scattered light, which indicates the quantity of NPs in the cells, was analyzed. The result shows an accurate size- and dose-dependent uptake of Au NPs (5, 30, 250 nm) in Caco-2 cells. The size- and dose- dependence of Au NPs (5, 30, 250 nm) and carbon NPs (50, 500 nm) in cells was validated by transmission electron microscope (TEM). This paper demonstrates the great potential of flow cytometry light scattering in the quantitative study of the size and dose effect on in situ metallic or non-metallic NPs in mammalian cells.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	13880764
Journal	Journal of Nanoparticle Research
Volume Number	19
Issue Number	2
e-ISSN	1572896X
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-02-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanoparticles Cells Flow cytometry Side scattered light Quantitative analysis Nanobiotechnology Nanotechnology Inorganic Chemistry Characterization and Evaluation of Materials Physical Chemistry Optics, Lasers, Photonics, Optical Devices
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Bioengineering Modeling and Simulation

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