NDLI: Hydrophobically modified chitosan/gold nanoparticles for DNA delivery

Content Provider	Springer Nature Link
Author	Bhattarai, Shanta Raj K.C., Remant Bahadur Aryal, Santosh Bhattarai, Narayan Kim, Sun Young Yi, Ho Keun Hwang, Pyoung Han Kim, Hak Yong
Copyright Year	2007
Abstract	Present study dealt an application of modified chitosan gold nanoparticles (Nac-6-Au) for the immobilization of necked plasmid DNA. Gold nanoparticles stabilized with N-acylated chitosan were prepared by graft-onto approach. The stabilized gold nanoparticles were characterized by different physico-chemical techniques such as UV-vis, TEM, ELS and DLS. MTT assay was used for in vitro cytotoxicity of the nanoparticles into three different cell lines (NIH 3T3, CT-26 and MCF-7). The formulation of plasmid DNA with the nanoparticles corresponds to the complex forming capacity and in-vitro/in-vivo transfection efficiency was studied via gel electrophoresis and transfection methods, respectively. Results showed the modified chitosan gold nanoparticles were well-dispersed and spherical in shape with average size around 10∼12 nm in triple distilled water at pH 7.4, and showed relatively no cytotoxicity at low concentration. Addition of plasmid DNA on the aqueous solution of the nanoparticles markedly reduced surface potential (50.0∼66.6%) as well as resulted in a 13.33% increase in hydrodynamic diameters of the formulated nanoparticles. Transfection efficiency of Nac-6-Au/DNA was dependent on cell type, and higher β-galactosidase activity was observed on MCF-7 breast cancer cell. Typically, this activity was 5 times higher in 4.5 mg/ml nanoparticles concentration than that achieved by the nanoparticles of other concentrations (and/or control). However, this activity was lower in in-vitro and dramatically higher in in-vivo than that of commercially available transfection kit (Lipofectin®) and DNA. From these results, it can be expected to develop alternative new vectors for gene delivery.
Starting Page	151
Ending Page	162
Page Count	12
File Format	PDF
ISSN	13880764
Journal	Journal of Nanoparticle Research
Volume Number	10
Issue Number	1
e-ISSN	1572896X
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-05-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chitosan DNA delivery Gene therapy Gold nanoparticles Non viral vectors Nanomedicine Applied Optics, Optoelectronics, Optical Devices Physical Chemistry Characterization and Evaluation of Materials Inorganic Chemistry Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Bioengineering Modeling and Simulation

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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