NDLI: Agar Diffusion Procedures for Susceptibility Testing of Malassezia pachydermatis: Evaluation of Mueller-Hinton Agar Plus 2 % Glucose and 0.5 µg/ml Methylene Blue as the Test Medium

Content Provider	Springer Nature Link
Author	Pasquetti, M. Chiavassa, E. Tizzani, P. Danesi, P. Pea, A.
Copyright Year	2015
Abstract	Aim of this study was to verify whether Mueller-Hinton agar supplemented with 2 % glucose and methylene blue (MH-GM), which is used for disk diffusion susceptibility testing of Candida species by the Clinical and Laboratory Standards Institute, is suitable for testing Malassezia pachydermatis. A variant of the disk diffusion procedure utilizing a 9-mm tablet was used to test 31 isolates against clotrimazole and miconazole using MH-GM as test medium. The MH-GM agar optimally supported the growth of all M. pachydermatis isolates, provided that the yeast inoculum was prepared with a lipid source (Tween 40 and 80). Zone edges were frequently definite and clear, facilitating the measurement of zone size and minimizing subjectivity. The inhibition zones correlated with MIC values obtained in a broth dilution assay. The agar diffusion method with MH-GM as the test medium appears as a suitable procedure for testing the susceptibility of M. pachydermatis to CTZ and MCZ in clinical laboratories. This test format may allow processing a large number of isolates in epidemiological studies. This may in turn facilitate clarifying to what extent the problem “drug resistance” accounts for cases of treatment failure in dogs with Malassezia otitis and dermatitis.
Starting Page	153
Ending Page	158
Page Count	6
File Format	PDF
ISSN	0301486X
Journal	Mycopathologia
Volume Number	180
Issue Number	3-4
e-ISSN	15730832
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Malassezia pachydermatis Susceptibility testing Agar Mueller-Hinton Dog Microbiology Medical Microbiology Eukaryotic Microbiology Microbial Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Veterinary Microbiology Applied Microbiology and Biotechnology Agronomy and Crop Science

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