NDLI: MiR-29a and miR-142-3p downregulation and diagnostic implication in human acute myeloid leukemia

Content Provider	Springer Nature Link
Author	Wang, Fang Wang, Xiao Shuang Yang, Gui Hua Zhai, Peng Fei Xiao, Zhen Xia, Liang Yu Chen, Li Rong Wang, Yu Wang, Xiao Zhong Bi, Lai Xi Liu, Nian Yu, Yang Gao, Da Huang, Bin Tao Wang, Jing Zhou, Dao Bin Gong, Jia Nan Zhao, Hua Lu Bi, Xiu Hua Yu, Jia Zhang, Jun Wu
Copyright Year	2011
Abstract	Expression profiling of microRNAs (miRNAs) in most diseases might be popular and provide the possibility for diagnostic implication, but few studies have accurately quantified the expression level of dysregulated miRNAs in acute myeloid leukemia (AML). In this study, we analyzed the peripheral blood mononuclear cells (PBMCs) from 10 AML patients (subtypes M1 to M5) and six normal controls by miRNA microarray and identified several differentially expressed miRNAs. Among them miR-29a and miR-142-3p were selectively encountered in Northern blot analysis and their significantly decreased expression in AML was further confirmed. Quantitative real-time PCR in 52 primarily diagnosed AML patients and 100 normal controls not only verified the expression properties of these 2 miRNAs, but also established that the expression level of miR-142-3p and miR-29a in PBMCs could be used as novel diagnostic markers. A better diagnostic outcome was achieved by combining miR-29a and miR-142-3p with about 90% sensitivity, 100% specificity, and an area under the ROC curve (AUC) of 0.97. Our results provide insights into the involvement of miRNAs in leukemogenesis, and offer candidates for AML diagnosis and therapeutic strategy.
Starting Page	2713
Ending Page	2722
Page Count	10
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	39
Issue Number	3
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-06-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	MicroRNAs (miRNAs) Acute myeloid leukemia (AML) Expression profile miR-29a miR-142-3p Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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