NDLI: Attenuation of cellular toxicity by calpain inhibitor induced by bacterial endotoxin: a mechanistic study using muscle precursor cells as a model system

Content Provider	Springer Nature Link
Author	Shang, Ke Zhang, Junfeng Amna, Touseef Yang, Jieun Cheng, Xiangchao Zhang, Chunjie Hwang, Inho
Copyright Year	2015
Abstract	This investigation was under taken to explore probable mechanisms and signal pathways involved in cytotoxicity induced by bacterial endotoxin lipopolysaccharide (LPS). Herein, we selected muscle precursor C2C12 myoblasts as representative cells to test effect of calpain inhibitor 3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid (PD150606) on LPS induced inflammation and apoptosis. In order to rule out the toxicity of endotoxin, mouse myoblasts were exposed to various concentrations of LPS and viability of cells and morphology were assessed using CCK-8 assay and simple microscopy respectively. Apoptotic cell death was examined by fluorescence microscope at regular time intervals. Additionally, LPS induced apoptosis in C2C12 cells were determined by mRNA expression of µ-calpain, caspase-3 and tumor necrosis factor alpha (TNF-α) and were quantified by qRT-PCR. Our results point out that LPS stimulation produced dose dependent toxicity in muscle precursor cells. Pre-treatment with a calpain inhibitor can significantly attenuate LPS-induced inflammation/apoptosis. Results of present research determined that mRNA expression of aforesaid genes was amplified (p < 0.05) in LPS stimulated C2C12 cells, whereas a noticeable drop off in mRNA expression of these genes was observed when pre-exposed with calpain inhibitor PD150606. Our study has outlined the current understanding regarding the connection between µ-calpain and caspase-3 in skeletal muscle wasting and as a result provides suitable choice for designing promising chemotherapeutic system for muscle illness and atrophy.
Starting Page	1281
Ending Page	1288
Page Count	8
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	42
Issue Number	8
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-03-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Skeletal muscle Endotoxin Inflammation Apoptosis Calpain Caspases Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A mechanistic investigation into non-infarcted brain injury induced by cerebral artery microemboli

Phorbol 12-myristate 13-acetate prevents apoptosis in erythroleukemia K562 cells induced by some nucleosides

Protective effect of calpain inhibitor N-acetyl-l-leucyl-l-leucyl-l-norleucinal on acute alcohol consumption related cardiomyopathy

Fibre type-specific expression of p94, a skeletal muscle-specific calpain

Skeletal muscle post-conditioning by diazoxide, anti-oxidative and anti-apoptotic mechanisms

Modulation of apoptosis-related cell signalling pathways by curcumin as a strategy to inhibit tumor progression

Progression of inflammation during immunodeficient mouse skeletal muscle regeneration

Curcumin induces apoptosis through mitochondrial pathway and caspases activation in human melanoma cells

Skeletal muscle regeneration induced by chorio-allantoic grafting

Attenuation of cellular toxicity by calpain inhibitor induced by bacterial endotoxin: a mechanistic study using muscle precursor cells as a model system

Similar Documents

A mechanistic investigation into non-infarcted brain injury induced by cerebral artery microemboli

Phorbol 12-myristate 13-acetate prevents apoptosis in erythroleukemia K562 cells induced by some nucleosides

Protective effect of calpain inhibitor N-acetyl-l-leucyl-l-leucyl-l-norleucinal on acute alcohol consumption related cardiomyopathy

Fibre type-specific expression of p94, a skeletal muscle-specific calpain

Skeletal muscle post-conditioning by diazoxide, anti-oxidative and anti-apoptotic mechanisms

Modulation of apoptosis-related cell signalling pathways by curcumin as a strategy to inhibit tumor progression

Progression of inflammation during immunodeficient mouse skeletal muscle regeneration

Curcumin induces apoptosis through mitochondrial pathway and caspases activation in human melanoma cells

Skeletal muscle regeneration induced by chorio-allantoic grafting

Attenuation of cellular toxicity by calpain inhibitor induced by bacterial endotoxin: a mechanistic study using muscle precursor cells as a model system