NDLI: Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway

Content Provider	Springer Nature Link
Author	Pang, Wei Jun Xiong, Yan Zhang, Zhao Wei, Ning Chen, Ni Yang, Gong she
Copyright Year	2012
Abstract	The effects of Sirt1 gene and resveratrol on porcine preadipocyte apoptosis have not been characterized. Here, we investigated the apoptotic effects of Sirt1 and resveratrol on porcine preadipocytes, finding that resveratrol-induced preadipocyte apoptosis and up-regulated protein levels of Sirt1. Intriguingly, Sirt1 knockdown by RNAi also resulted in preadipocyte apoptosis. Combining resveratrol treatment and Sirt1 knockdown has an additive effect to promote porcine preadipocyte death. We found that resveratrol treatment alone dose-dependently increased caspase-3 cleavage, as well as the levels of Bax, p53 and acetylated-p53. Interestingly, the ratio of acetylated-p53 over p53 was declined owing to deacetylation by increased Sirt1 expression. Down-regulation of Sirt1 also elevated the cleavage of caspase-3 with a decrease of p53 acetylation. These data indicate that although resveratrol treatment up-regulates Sirt1 expression, it augments porcine preadipocyte apoptosis in a Sirt1-independent manner. The regulation of apoptosis by resveratrol and Sirt1 may provide a novel insight to control preadipocyte number through cellular apoptosis.
Starting Page	129
Ending Page	139
Page Count	11
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	40
Issue Number	1
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-10-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sirt1 Resveratrol Pig Preadipocyte Apoptosis Animal Anatomy / Morphology / Histology Animal Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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