NDLI: Mutations in the passenger polypeptide can affect its partitioning between mitochondria and cytoplasm : Mutations can impair the mitochondrial import of DsRed

Content Provider	Springer Nature Link
Author	Pastukh, Viktoriya Shokolenko, Inna N. Wilson, Glenn L. Alexeyev, Mikhail F.
Copyright Year	2007
Abstract	In this study, we report that the partitioning between mitochondria and cytoplasm of two variants, mCherry and DsRed Express (DRE), of the red fluorescent protein, DsRed, fused to one of the six matrix targeting sequences (MTSs) can be affected by both MTS and amino acid substitutions in DsRed. Of the six MTSs tested, MTSs from superoxide dismutase and DNA polymerase gamma failed to direct mCherry, but not DRE to mitochondria. By evaluating a series of chimeras between mCherry and DRE fused to the MTS of superoxide dismutase, we attribute the differences in the mitochondrial partitioning to differences in the primary amino acid sequence of the passenger polypeptide. The impairment of mitochondrial partitioning closely parallels the number of mCherry-specific mutations, and is not specific to mutations located in any particular region of the polypeptide. These observations suggest that both MTS and the passenger polypeptide affect the efficiency of mitochondrial import and provide a rationale for the observed diversity in the primary amino acid sequences of natural MTSs.
Starting Page	215
Ending Page	223
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	35
Issue Number	2
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-03-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DsRed express DsRed express-mCherry chimera DsRed mutant mCherry Mitochondrial import Protein maturation Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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