NDLI: Sequence characterization and promoter identification of porcine APC10 gene

Content Provider	Springer Nature Link
Author	He, W. B. Wang, Z. W. Li, Y. Tang, Z. L. Yang, S. L. Mu, Y. L. Peng, K. M. Li, K.
Copyright Year	2010
Abstract	APC10 protein, a subunit of the anaphase-promoting complex (APC), plays an essential role in the progression of cells from mitosis to G1. In this study, we cloned and sequenced partial cDNA, intron 1 and 5′-flanking sequences of porcine APC10. The partial cDNA is 595 bp long and has an open reading frame of 558 bp which encodes 185 putative animo acids. Real-time PCR analysis revealed that the porcine APC10 mRNA expression shows a wide distribution and expression levels varies within a small different range in detected tissues. The deduced protein has a high identity with other eight species and comprises a conserved DOC domain. The phylogenetic tree indicated that porcine APC10 has the closest genetic relationship with human, monkey and dog. Promoter activity was demonstrated by transient transfection of 5′-deletion promoter/luciferase constructs into PK15 cells, and indicated that the proximal region from −2,052 to −1,764 is necessary for basal promoter activity. Positive cis-regulatory elements are present from −2,544 to −2,052 and from −3,114 to −2,774, while negative cis-regulatory elements may be present from −2,774 to −2,544. Yeast one-hybrid assay revealed Sp1 can interact with proximal promoter region of porcine APC10.
Starting Page	3841
Ending Page	3849
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	37
Issue Number	8
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anaphase-promoting complex Promoter TAIL-polymerase chain reaction Yeast one-hybrid assay Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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