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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kang, Bo Hong, Jiang Xiao, Jian Zhu, Xiaoyan Ni, Xin Zhang, Yufeng He, Bin Wang, Zhing |
| Copyright Year | 2014 |
| Abstract | The protective effect of hydrogen sulfide (H$_{2}$S) against myocardial ischemia/reperfusion (IR) injury via anti-apoptotic signaling is well established, but the underlying mechanism remains unclear. Recently, miRNAs have been identified as important mediators of myocardial injury by regulating apoptosis-related genes. It was found in our previous preliminary study that microRNA-1 (miR-1) expression underwent a significant change in IR group compared to H$_{2}$S preconditioned group, indicating that miR-1 possessed myocyte-specific properties. In the present study, we intended to see whether miR-1 participated in H$_{2}$S protection of cardiomyocytes against IR-induced apoptosis by regulating apoptosis-related genes. Cardiomyocytes of neonatal rats were subjected to hypoxia/reoxygenation (HR) injury with or without H$_{2}$S preconditioning, while the myocardium of adult SD rats was subjected to IR with or without H$_{2}$S preconditioning. It was found that HR injury increased apoptosis of cardiac myocytes, up-regulated the expression of miR-1, and down-regulated the expression of Bcl-2. H$_{2}$S preconditioning attenuated cardiomyocyte apoptosis and LDH release, as well as enhanced cell viability following HR injury. MiR-1 was up-regulated by HR and down-regulated by H$_{2}$S preconditioning. In contrast, Bcl-2 was down-regulated by HR and up-regulated by H$_{2}$S preconditioning. In addition, Bcl-2 protein was down-regulated by the miR-1 mimic in a dose-dependent manner. H$_{2}$S also attenuated IR-induced cardiomyocyte apoptosis in vivo. MiR-1 regulated H$_{2}$S protection of cardiomyocytes against IR-induced apoptosis by stimulating Bcl-2. These results implicate miR-1 as an important regulator of H$_{2}$S on the IR myocardium. |
| Starting Page | 6845 |
| Ending Page | 6853 |
| Page Count | 9 |
| File Format | |
| ISSN | 03014851 |
| Journal | Molecular Biology Reports |
| Volume Number | 41 |
| Issue Number | 10 |
| e-ISSN | 15734978 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2014-07-10 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | MicroRNA-1 Apoptosis Hydrogen sulfide Cardiomyocyte Ischemia/reperfusion Animal Biochemistry Animal Anatomy / Morphology / Histology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Molecular Biology |
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