NDLI: BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis

Content Provider	Springer Nature Link
Author	Jiang, Jingjing Yu, Youjian Dong, Heng Yao, Lina Zhang, Zhixian Cao, Jiashu
Copyright Year	2013
Abstract	Brassica campestris Male Fertility 21 (BcMF21) was previously isolated from the flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. Here, we used antisense RNA technology to knock down the expression level of BcMF21 in B. campestris and analyzed the phenotype of the transgenic plants. Alexander staining and scanning electron microscope revealed sterility and exine deformities in the mature pollen grains of BcMF21 antisense RNA transgenic plants. The germination furrow of the BcMF21 antisense RNA transgenic pollen was covered by lipid like materials. The pollen tubes burst and could not grow normally in vitro. Therefore, we presented here BcMF21 might be an important gene for pollen development and germination.
Starting Page	537
Ending Page	544
Page Count	8
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	1
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brassica campestris Brassica rapa Chinese cabbage BcMF21 Exine Microspore development Pollen Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Sequence characterization and expression pattern of BcMF21, a novel gene related to pollen development in Brassica campestris ssp. chinensis

BcMF13, a new reproductive organ-specific gene from Brassica rapa. ssp. chinensis, affects pollen development

A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi

BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp. chinensis

Characterization of a putative pollen-specific arabinogalactan protein gene, BcMF8, from Brassica campestris ssp. chinensis

Characterization of a male sterile related gene BcMF15 from Brassica campestris ssp. chinensis

Characterization of BcMF23a and BcMF23b, two putative pectin methylesterase genes related to pollen development in Brassica campestris ssp. chinensis

Molecular cloning and characterization of a novel pollen predominantly membrane protein gene BcMF12 from Brassica campestris ssp. chinensis

BcMF5, a pollen coat protein gene (PCP), from Brassica rapa. ssp. chinensis, involved in the transcription of different lengths of 3′-UTRs of PCPs

BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis

Similar Documents

Sequence characterization and expression pattern of BcMF21, a novel gene related to pollen development in Brassica campestris ssp. chinensis

BcMF13, a new reproductive organ-specific gene from Brassica rapa. ssp. chinensis, affects pollen development

A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi

BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp. chinensis

Characterization of a putative pollen-specific arabinogalactan protein gene, BcMF8, from Brassica campestris ssp. chinensis

Characterization of a male sterile related gene BcMF15 from Brassica campestris ssp. chinensis

Characterization of BcMF23a and BcMF23b, two putative pectin methylesterase genes related to pollen development in Brassica campestris ssp. chinensis

Molecular cloning and characterization of a novel pollen predominantly membrane protein gene BcMF12 from Brassica campestris ssp. chinensis

BcMF5, a pollen coat protein gene (PCP), from Brassica rapa. ssp. chinensis, involved in the transcription of different lengths of 3′-UTRs of PCPs

BcMF21 is important for pollen development and germination in Brassica campestris ssp. chinensis