NDLI: Role of the functional variant (−652T>G) in the XRCC4 promoter in prostate cancer

Content Provider	Springer Nature Link
Author	Shao, Ning Li, JiuMing Xu, Bin Wang, Yang Lu, XiaoJie Feng, NingHan
Copyright Year	2014
Abstract	Several genes encoding DNA repair molecules have been proposed as cancer-susceptibility genes. Many studies have suggested that SNPs in XRCC4 could be implicated in altering the risk of prostate cancer (PCa). We examined the role of the functional variant (−652T>G) in the XRCC4 promoter in PCa. The transcriptional activity of XRCC4 gene was measured by luciferase assay. We performed real-time PCR/immunohistochemical assay to verify the association between expression level of XRCC4 mRNA/protein and XRCC4 −652T>G polymorphism. In addition, electrophoretic mobility shift assay (EMSA) was used to confirm whether this polymorphism has an effect on binding ability of the transcription factor. We found that the G variant significantly increased the transcription activity of the XRCC4 gene and the binding ability of transcriptional factor GATA-1 to the XRCC4 promoter. Furthermore, the results suggested that the XRCC4 protein and mRNA were overexpressed in individuals who carried the −652G allele compared to carriers of the −652T allele. In addition, the expression of XRCC4 in PCa tissues was lower than in adjacent normal tissues. Our data suggest that the XRCC4 promoter −652G>T polymorphism is functional and may influence genetic susceptibility to prostate cancer. Case–control studies are required to validate our findings in the future.
Starting Page	7463
Ending Page	7470
Page Count	8
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	11
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-08-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	XRCC4 EMSA Prostate cancer Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Gene variants of XRCC4 and XRCC3 and their association with risk for urothelial bladder cancer

Interleukin-6 gene −174G>C and −636G>C promoter polymorphisms and prostate cancer risk

Association of HSP70-hom genetic variant with prostate cancer risk

Molecular cloning and preliminary analysis of the human α-methylacyl-CoA racemase promoter

Significance of Tumor Necrosis factor α-308 (G/A) gene polymorphism in the development of prostate cancer

Cloning and characterization of the 5′-flanking region of the pig AgRP gene

CDH1 promoter polymorphism and stomach cancer susceptibility

The Ku70 −1310C/G promoter polymorphism is associated with breast cancer susceptibility in Chinese Han population

The association between KL polymorphism and prostate cancer risk in Korean patients

Role of the functional variant (−652T>G) in the XRCC4 promoter in prostate cancer

Similar Documents

Gene variants of XRCC4 and XRCC3 and their association with risk for urothelial bladder cancer

Interleukin-6 gene −174G>C and −636G>C promoter polymorphisms and prostate cancer risk

Association of HSP70-hom genetic variant with prostate cancer risk

Molecular cloning and preliminary analysis of the human α-methylacyl-CoA racemase promoter

Significance of Tumor Necrosis factor α-308 (G/A) gene polymorphism in the development of prostate cancer

Cloning and characterization of the 5′-flanking region of the pig AgRP gene

CDH1 promoter polymorphism and stomach cancer susceptibility

The Ku70 −1310C/G promoter polymorphism is associated with breast cancer susceptibility in Chinese Han population

The association between KL polymorphism and prostate cancer risk in Korean patients

Role of the functional variant (−652T>G) in the XRCC4 promoter in prostate cancer