NDLI: Cloning, Expression and Characterization of the Human NOB1 gene

Content Provider	Springer Nature Link
Author	Zhang, Yue Ni, Jun Zhou, Guangjin Yuan, Jian Ren, Weihua Shan, Yuxi Tang, Wenwen Yu, Long Zhao, Shouyuan
Copyright Year	2005
Abstract	The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.
Starting Page	185
Ending Page	189
Page Count	5
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	32
Issue Number	3
e-ISSN	15734978
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	NOB1 PIN domain subcellular localization tissue expression pattern zinc ribbon domain Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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