NDLI: Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1α

Content Provider	Springer Nature Link
Author	Koo, Ja Hyun Yoon, Heeseok Kim, Won Ju Lim, Sangho Park, Hong Jai Choi, Je Min
Copyright Year	2014
Abstract	Cytokines are released from the cell, bind to their receptors, and affect cellular responses. The precursor form of interleukin 1 alpha (pIL-1α) has a nuclear localization sequence (NLS) that causes it to be localized to the nucleus and regulate specific gene expression. The amino acids of the NLS are basic amino acid-rich sequences, as is the cell penetrating peptide (CPP), which has been widely studied as a way to deliver macromolecules into cells. Here, we hypothesized that the NLS in pIL-1α (pIL-1αNLS) can penetrate the cell membrane and it could deliver macromolecules such as protein in vivo. We characterized cell membrane penetration ability of pIL-1αNLS or its tandem repeated form (2pIL-1αNLS) to enhance its intracellular delivery efficiency. 2pIL-1αNLS showed comparable protein delivery efficiency to TAT–CPP and it mediates endocytosis following heparan sulfate interaction. 2pIL-1αNLS conjugated enhanced green fluorescence protein was localized to the nucleus and the cytoplasm. Intra-peritoneal administration of 2pIL-1αNLS conjugated dTomato protein showed remarkable in vivo intracellular delivery efficiency in various tissues including spleen, liver, and intestine in mice. Moreover, cytotoxicity of 2pIL-1αNLS was not observed even at 100 μM. Our results demonstrate cell membrane-penetrating function of NLS in pIL-1α, which can be used as a safe therapeutic macromolecular delivery peptide.
Starting Page	8117
Ending Page	8126
Page Count	10
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	12
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-09-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	IL-1α Cytokine Nuclear localization sequence Cell penetrating peptide Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A novel human derived cell-penetrating peptide in drug delivery

The function of activatable cell-penetrating peptides in human intrahepatic bile duct epithelial cells

Human Protein 53-Derived Cell-Penetrating Peptides

Specific induction of Z-DNA conformation by a nuclear localization signal peptide of lupin glutaminyl tRNA synthetase

Primary function analysis of human mental retardation related gene CRBN

Intracellular localization and domain organization of human TRIM41proteins

Systematic analysis of gene expression level with tissue-specificity, function and protein subcellular localization in human transcriptome

Sequence of the human CALM II calmodulin gene promoter region

Design of a Tumor Homing Cell-Penetrating Peptide for Drug Delivery

Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1α

Similar Documents

A novel human derived cell-penetrating peptide in drug delivery

The function of activatable cell-penetrating peptides in human intrahepatic bile duct epithelial cells

Human Protein 53-Derived Cell-Penetrating Peptides

Specific induction of Z-DNA conformation by a nuclear localization signal peptide of lupin glutaminyl tRNA synthetase

Primary function analysis of human mental retardation related gene CRBN

Intracellular localization and domain organization of human TRIM41proteins

Systematic analysis of gene expression level with tissue-specificity, function and protein subcellular localization in human transcriptome

Sequence of the human CALM II calmodulin gene promoter region

Design of a Tumor Homing Cell-Penetrating Peptide for Drug Delivery

Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1α