NDLI: Cadmium induces cadmium-tolerant gene expression in the filamentous fungus Trichoderma harzianum

Content Provider	Springer Nature Link
Author	Cacciola, Santa O. Puglisi, Ivana Faedda, Roberto Sanzaro, Vincenzo Pane, Antonella Lo Piero, Angela R. Evoli, Maria Petrone, Goffredo
Copyright Year	2015
Abstract	The filamentous fungus Trichoderma harzianum, strain IMI 393899, was able to grow in the presence of the heavy metals cadmium and mercury. The main objective of this research was to study the molecular mechanisms underlying the tolerance of the fungus T. harzianum to cadmium. The suppression subtractive hybridization (SSH) method was used for the characterization of the genes of T. harzianum implicated in cadmium tolerance compared with those expressed in the response to the stress induced by mercury. Finally, the effects of cadmium exposure were also validated by measuring the expression levels of the putative genes coding for a glucose transporter, a plasma membrane ATPase, a Cd$^{2+}$/Zn$^{2+}$ transporter protein and a two-component system sensor histidine kinase YcbA, by real-time-PCR. By using the aforementioned SSH strategy, it was possible to identify 108 differentially expressed genes of the strain IMI 393899 of T. harzianum grown in a mineral substrate with the addition of cadmium. The expressed sequence tags identified by SSH technique were encoding different genes that may be involved in different biological processes, including those associated to primary and secondary metabolism, intracellular transport, transcription factors, cell defence, signal transduction, DNA metabolism, cell growth and protein synthesis. Finally, the results show that in the mechanism of tolerance to cadmium a possible signal transduction pathway could activate a Cd$^{2+}$/Zn$^{2+}$ transporter protein and/or a plasma membrane ATPase that could be involved in the compartmentalization of cadmium inside the cell.
Starting Page	1559
Ending Page	1570
Page Count	12
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	42
Issue Number	11
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Heavy metals Cadmium tolerance SSH strategy Ascomycetes Real-time PCR Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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