NDLI: Molecular cloning and differential expression in tissues of a tyrosinase gene in the Pacific oyster Crassostrea gigas

Content Provider	Springer Nature Link
Author	Yu, Xue Yu, Hong Kong, Lingfeng Guo, Fengguang Zhu, Guan Li, Qi
Copyright Year	2014
Abstract	Tyrosinases are a group of type-3 copper proteins that catalyze the first two reactions in the melanin biosynthesis in organisms ranging from bacteria, fungi, plants to animals. Tyrosinases are not only involved in pigmentation, but also play an important role in the innate immunity in invertebrates. Additionally, tyrosinases are also known to be involved in the biogenesis and pigmentation of shells. The recently published Crassostrea gigas genome sequences revealed that the Pacific oyster possesses at least 26 tyrosinase isoforms. However, their molecular features are largely understudied. In fact, the full-length mRNA sequence was determined for one of the tyrosinase genes (i.e., CgTry1; aka cgi-tyr1). Here we report the full-length transcript of a second C. gigas tyrosinase (CgTyr2) sequence and the determination of its sequence features characteristic to the tyrosinase family proteins. We also showed that CgTyr2 gene was differentially expressed with the highest level of expression in mantle edges, suggesting its potential role in the formation of periostracum/pigmentation. Our comprehensive phylogenetic reconstructions supported that hemocyanins possibly evolved from a tyrosinase by an ancient gene duplication followed by functional differentiation, and the current large number of tyrosinase isoforms in C. gigas and other mollusks were originated from multiple gene duplication events that took places before and after mollusk species were established.
Starting Page	5403
Ending Page	5411
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	8
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-05-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Crassostrea gigas Tyrosinase Pigmentation Gene expression Molecular phylogeny Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas

Development and characterization of microsatellite markers for the Pacific Oyster Crassostrea gigas

Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas

An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation

Eleven polymorphic simple sequence repeat markers from expressed sequence tags of Pacific oyster Crassostrea gigas EST database

Identification and functional characterization of MRE-binding transcription factor (MTF) in Crassostrea gigas and its conserved role in metal-induced response

Pigmentation in Black-boned sheep (Ovis aries): association with polymorphism of the Tyrosinase gene

Molecular cloning, characterization and differential expression of Cdc42 in Fonsecaea monophora

Molecular cloning of two molluscan caspases and gene functional analysis during Crassostrea angulata (Fujian oyster) larval metamorphosis

Molecular cloning and differential expression in tissues of a tyrosinase gene in the Pacific oyster Crassostrea gigas

Similar Documents

In silico cloning, expression of Rieske-like apoprotein gene and protein subcellular localization in the Pacific oyster, Crassostrea gigas

Development and characterization of microsatellite markers for the Pacific Oyster Crassostrea gigas

Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas

An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation

Eleven polymorphic simple sequence repeat markers from expressed sequence tags of Pacific oyster Crassostrea gigas EST database

Identification and functional characterization of MRE-binding transcription factor (MTF) in Crassostrea gigas and its conserved role in metal-induced response

Pigmentation in Black-boned sheep (Ovis aries): association with polymorphism of the Tyrosinase gene

Molecular cloning, characterization and differential expression of Cdc42 in Fonsecaea monophora

Molecular cloning of two molluscan caspases and gene functional analysis during Crassostrea angulata (Fujian oyster) larval metamorphosis

Molecular cloning and differential expression in tissues of a tyrosinase gene in the Pacific oyster Crassostrea gigas