NDLI: Expressing murine p56Hck$^{ca}$ promotes HeLa cells’ motility and invasion via triggering redistribution of F-actin and microtubules

Content Provider	Springer Nature Link
Author	Gong, Jie Yan, Jin chuan Gu, Hai yong Kong, Xiang qing Cao, Ke jiang
Copyright Year	2012
Abstract	Hck is the unique example among the Src PTKs to be expressed as two isoforms, which are generated by alternative translation. The two isoforms differs from each other by a 21 N-terminal amino acids sequence which supports myristoylation. Though it has been shown that these different acylation states govern the different subcellular localization of the isoforms and each Hck isoform could play a specific role, little study focus on the function of p56Hck. To investigated the role of p56Hck isoform in cell migration, GFP targeted p56Hck plasmid and its constitutively active form were constructed and transiently transfected into HeLa cells, F-actin staining and Indirect immunofluorescence for microtubules were then performed. Phagokinetic track motility assay and In vitro invasion assays were also investigated after transiently transfection respectively. In this study, we found ectopically expressing a constitutively active form of 56Hck will lead to membrane protrusion and F-actin reorganization in HeLa cells. Both 56Hck and its constitutive active form will lead to redistribution of microtubules and enhancement of cell motility and cell invasion. Hck inhibitor PP2 supplementation eliminated cell motility and cell invasion of p56Hck while PP3, a negative control of PP2 didn’t eliminate cell motility and cell invasion of p56Hck. It is indicated that enhanced cell motility and cell invasion in p56Hck ectopically expressed HeLa cells are the results of reorganization of F-actin and microtubules.
Starting Page	6521
Ending Page	6527
Page Count	7
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	39
Issue Number	6
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	p56Hck HeLa cells Membrane protrusion Cell motility Cell invasion Animal Anatomy / Morphology / Histology Animal Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Testosterone promotes vascular endothelial cell migration via upregulation of ROCK-2/moesin cascade

Zebularine inhibits the growth of HeLa cervical cancer cells via cell cycle arrest and caspase-dependent apoptosis

Computational prediction of actin–actin interaction

Stat3 promotes invasion of esophageal squamous cell carcinoma through up-regulation of MMP2

Lipopolysaccharide promotes adhesion and invasion of hepatoma cell lines HepG2 and HepG2.2.15

Suberoyl bishydroxamic acid-induced apoptosis in HeLa cells via ROS-independent, GSH-dependent manner

Ca$^{2+}$-dependent actin remodeling in the contracting A7r5 cell

Osteopontin promotes hepatocellular carcinoma invasion by up-regulating MMP-2 and uPA expression

A murine TSPY

Expressing murine p56Hck$^{ca}$ promotes HeLa cells’ motility and invasion via triggering redistribution of F-actin and microtubules

Similar Documents

Testosterone promotes vascular endothelial cell migration via upregulation of ROCK-2/moesin cascade

Zebularine inhibits the growth of HeLa cervical cancer cells via cell cycle arrest and caspase-dependent apoptosis

Computational prediction of actin–actin interaction

Stat3 promotes invasion of esophageal squamous cell carcinoma through up-regulation of MMP2

Lipopolysaccharide promotes adhesion and invasion of hepatoma cell lines HepG2 and HepG2.2.15

Suberoyl bishydroxamic acid-induced apoptosis in HeLa cells via ROS-independent, GSH-dependent manner

Ca$^{2+}$-dependent actin remodeling in the contracting A7r5 cell

Osteopontin promotes hepatocellular carcinoma invasion by up-regulating MMP-2 and uPA expression

A murine TSPY

Expressing murine p56Hck$^{ca}$ promotes HeLa cells’ motility and invasion via triggering redistribution of F-actin and microtubules