NDLI: Prediction and validation of conservative microRNAs of Solanum tuberosum L.

Content Provider	Springer Nature Link
Author	Yang, Wenzheng Liu, Xin Zhang, Jianguang Feng, Junli Li, Chao Chen, Jishuang
Copyright Year	2009
Abstract	Potato (Solanum tuberosum) is an important crop around the world, and accounts for a significant amount of the food consumed by humans. However, little information is available about potato miRNAs which play important regulatory roles in plant growth and development. In the present study, computational prediction of potential miRNAs from potato revealed 71 miRNAs belonging to 48 families. Amongst these 71 mRNAs, 65 were predicted for the first time. Most potato miRNA families have one to three members, and sequence analysis showed that the candidate pre-miRNA sequences varied from 48 to 224 bp in length. To verify the predicted miRNAs, specific stem-loop RT primers were designed and real-time PCR assays were used to profile the expression levels of seven miRNAs from different tissues of potato. The results showed that all the selected miRNAs were successfully amplified. Most of them had their highest expression levels in leaves, and the lowest levels in the stem, while miR159 and miR164 presented a different expression pattern. The specific expression levels of each miRNAs in the tested tissues may be related to their particular functions in regulating potato vegetative growth and organ development.
Starting Page	3081
Ending Page	3087
Page Count	7
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	37
Issue Number	7
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-10-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Solanum tuberosum miRNA Prediction Stem-loop real-time RT–PCR Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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