NDLI: Sequence polymorphisms in Zmisa2 gene are significantly associated with starch pasting and gelatinization properties in maize (Zea mays L.)

Content Provider	Springer Nature Link
Author	Yang, Zefeng Zhang, Enying Jiang, Ying Xu, Shuhui Pan, Liang Chen, Qing Xu, Chenwu
Copyright Year	2014
Abstract	Researches on the genetic basis of starch pasting and gelatinization properties will provide foundation of maize improvement for quality, feed and industrial applications. Maize gene Zmisa2, encoding an isoamylase-type starch debranching enzyme, plays important roles in starch biosynthesis. In this study, the genomic sequences of the gene Zmisa2 in 72 elite maize inbred lines were obtained, and the nucleotide polymorphisms and haplotype diversity were detected. In addition, seven pasting and four gelatinization properties of maize were measured for the tested inbred lines using rapid visco analyzer and differential scanning calorimeter, respectively. A total of 99 sequence variants, including 91 SNPs and 8 indels, were identified at the promoter and coding regions of this gene. Although the frequency of polymorphism in promoter region is much higher than that of coding region, the SNPs in the coding region of maize gene Zmisa2 classified this gene into 21 haplotypes, which encode 11 different ISA2 proteins. Furthermore, the association of the variants of Zmisa2 gene with maize starch pasting and gelatinization properties was estimated, and the results revealed that seven SNPs in coding region, including four nonsynonymous sites, were significantly associated with phenotypic variations of pasting time and enthalpy of transition (ΔH). These results suggested that the polymorphism in maize Zmisa2 locus could be used in molecular marker-assisted selection for improvement of quality in maize breeding programs.
Starting Page	1833
Ending Page	1842
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	34
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Maize Starch pasting and gelatinization properties Zmisa2 gene Sequence polymorphism Association analysis Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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