NDLI: Particle bombardment and the genetic enhancement of crops: myths and realities

Content Provider	Springer Nature Link
Author	Altpeter, Fredy Baisakh, Niranjan Beachy, Roger Bock, Ralph Capell, Teresa Christou, Paul Daniell, Henry Datta, Karabi Datta, Swapan Dix, Philip J. Fauquet, Claude Huang, Ning Kohli, Ajay Mooibroek, Hans Nicholson, Liz Nguyen, Thi Thanh Nugent, Gregory Raemakers, Krit Roma, Andrea Somers, David A. Stoger, Eva Taylor, Nigel Visser, Richard
Copyright Year	2005
Abstract	DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, single-copy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions.
Starting Page	305
Ending Page	327
Page Count	23
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	15
Issue Number	3
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Crops Genetic engineering Particle bombardment Transformation Transgene expression Transgene structure Transgenic plants Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants

Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment

Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment

Efficient and stable transformation of hop (Humulus lupulus L.) var. Eroica by particle bombardment

Transformation of Plants with Multiple Cassettes Generates Simple Transgene Integration Patterns and High Expression Levels

The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat

Genetic transformation of Alstroemeria using particle bombardment

Marker-free transgenic corn plant production through co-bombardment

Expression and inheritance of foreign genes in transgenic peanut plants generated byAgrobacterium-mediated transformation

Particle bombardment and the genetic enhancement of crops: myths and realities

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