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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ryynänen, Leena Sillanpää, Maarit Kontunen Soppela, Sari Tiimonen, Heidi Kangasjärvi, Jaakko Vapaavuori, Elina Häggman, Hely |
| Copyright Year | 2002 |
| Abstract | The aim of the study was to develop a preservation method for transgenic silver birch (Betula pendula Roth) lines based on cryopreservation. Specific attention was paid to transgene stability and functioning. Vegetative buds collected from one- or two-year-old silver birches representing four transgenic lines and two wild-type lines were used as explants. Generally, the average regeneration of either transgenic or wild-type, cryoperserved and non-cryopreserved control buds was excellent, and varied from 72 to 100 percent. The regeneration percentage of cryopreserved buds was, however, significantly lower than that of non-cryopreserved control buds when estimated two weeks after thawing, but the differences were no longer significant four weeks after thawing. Growth of the plants in the greenhouse was more dependent on the clone than on the cryopreservation treatment. The studied transgenic lines have three (line E/5) to nine (line R/3.2) copies of transferred neomycin phosphotransferase genes that were also found to be stable after cryopreservation. In general, the neomycin phosphotransferase transcript levels did not change due to cryopreservation. The results indicate that it is possible to apply the cryopreservation technique to preserve valuable transgenic lines of a forest tree, silver birch. The method presented here leads to high regeneration percentages combined with transgene stability and functioning. |
| Starting Page | 143 |
| Ending Page | 152 |
| Page Count | 10 |
| File Format | |
| ISSN | 13803743 |
| Journal | Molecular Breeding |
| Volume Number | 10 |
| Issue Number | 3 |
| e-ISSN | 15729788 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2002-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science |
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