NDLI: Identification of a single nucleotide deletion causing frameshift mutation in OsDFR2A in a genic male sterile mutant of rice and its possible application to F$

Content Provider	Springer Nature Link
Author	Shirasawa, Sachiko Kifuji, Yasuko Komiya, Reina Kitashiba, Hiroyasu Nishimura, Miru Nishio, Takeshi
Copyright Year	2013
Abstract	Stable genic male sterility (GMS), which is not influenced by environmental factors, has not been used for F$_{1}$ hybrid seed production because male-sterile inbred lines cannot be developed and male-sterile plants must be selected from segregating populations every time. However, the stability of male sterility may provide a reliable system for F$_{1}$ seed production without contamination of selfed seeds. A genic male-sterile mutant in rice (Oryza sativa L.), C204, which was selected from progeny of the cultivar ‘Koshihikari’ irradiated by gamma rays, has shorter and whiter anthers than those of ‘Koshihikari’ and has no pollen grains. Segregation analysis of C204 suggested the male sterility of this mutant to be controlled by a recessive allele of a single gene. Linkage analysis of a mutated gene responsible for the male sterility revealed the gene to be in a region of ca. 75 kb on the long arm of chromosome 9. The nine genes predicted in the 75-kb region were sequenced, and compared with the published Nipponbare genome sequences. A single-base deletion was found in the first exon of a C204 allele of Os09g0493500, which encodes an NAD-dependent epimerase/dehydratase family protein, resulting in a frameshift causing a premature stop codon. A dot-blot single nucleotide polymorphism marker for detection of the single-base deletion in Os09g0493500 was developed. We herein propose an F$_{1}$ hybrid seed production system using stable GMS with a simple selection method of GMS plants.
Starting Page	805
Ending Page	814
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	31
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-01-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Stable GMS Fine mapping NAD-dependent epimerase/dehydratase Dot-blot-SNP marker DNA marker selection Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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