NDLI: Molecular differentiation of null alleles at ZCCT-1 genes on the A, B, and D genomes of hexaploid wheat

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Molecular differentiation of null alleles at ZCCT-1 genes on the A, B, and D genomes of hexaploid wheat

Content Provider	Springer Nature Link
Author	Zhu, Xinkai Tan, ChorTee Cao, Shuanghe Yan, Liuling
Copyright Year	2010
Abstract	A dominant allele of the vernalization gene Vrn-2 is the wild type conferring winter growth habit, whereas a recessive vrn-2 allele confers spring growth habit. The recessive vrn-2 allele is mutated due to the deletion of the complete gene (a null form) or alternation of a key amino acid in the VRN-2 protein (a nonfunctional form) in diploid wheat or tetraploid wheat. VRN-2 is also denoted ZCCT due to the presence of a zinc finger and a CCT domain in its protein. There are two paralogous ZCCT genes at the VRN-2 locus in diploid Triticum monococcum and three paralogous ZCCT genes on each of the A and B genomes in tetraploid wheat, but little is known about the allelic variation in VRN-2 in hexaploid wheat. In the study reported here, we performed a one-shot PCR to simultaneously amplify the promoter regions of the three ZCCT-1 genes from hexaploid wheat, including the 302-bp fragment from ZCCT-A1, the 294-bp fragment from ZCCT-B1, and the 320-bp fragment from ZCCT-D1. Each amplicon could be differentiated by electrophoresis in an acrylamide/bisacrylamide gel. This PCR marker for different lengths of the three ZCCT-1 genes was used to search for null alleles in hexaploid wheat. A null allele was found in each of ZCCT-A1, ZCCT-B1, and ZCCT-D1 among 74 cultivars and genetic stocks of U.S. hexaploid wheat. Among 54 Chinese wheat cultivars, breeding lines, and landraces, we identified three accessions carrying a single null allele at ZCCT-A1, three accessions carrying a null allele at ZCCT-B1, and one accession carrying a double null allele at both ZCCT-A1 and ZCCT-D1. The potential application of these natural ZCCT-1 mutant materials in wheat breeding programs and studies on the genetics of wheat is discussed.
Starting Page	501
Ending Page	510
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	27
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-05-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Growth habit Natural mutants Polyploidy genomes Vernalization VRN-2 genes Wheat genome evolution Plant Physiology Biotechnology Plant Genetics & Genomics Plant Sciences Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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Molecular characterization of the allelic variation at the VRN-H2 vernalization locus in barley

Duplicated, deleted and translocated VRN2 genes in hexaploid wheat

Distribution of different Vrn-B1 alleles in hexaploid spring wheat germplasm

Molecular characterization of the allelic variation at the VRN-H2 vernalization locus in barley

Distribution of the Vrn-D1b allele associated with facultative growth habit in Chinese wheat accessions

Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat

Haplotype analysis of vernalization loci in European barley germplasm reveals novel VRN-H1 alleles and a predominant winter VRN-H1/VRN-H2 multi-locus haplotype

Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat

Molecular-genetic mechanisms of regulation of growth habit in wheat

Molecular differentiation of null alleles at ZCCT-1 genes on the A, B, and D genomes of hexaploid wheat

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Molecular characterization of the allelic variation at the VRN-H2 vernalization locus in barley

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Distribution of different Vrn-B1 alleles in hexaploid spring wheat germplasm

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Molecular differentiation of null alleles at ZCCT-1 genes on the A, B, and D genomes of hexaploid wheat