NDLI: Marker-assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers

Content Provider	Springer Nature Link
Author	Hansen, Mats Halldén, Christer Nilsson, Nils Otto Säll, Torbjörn
Copyright Year	1997
Abstract	Bulked segregant analysis was used to identify RAPD markers in oilseed rape (Brassica napus L.) that were linked to a male fertility restorer gene for Ogura cytoplasmic male sterility. After screening for polymorphisms using 960 primers, 14 RAPD markers were mapped to a 25 cM region including the restorer locus, a mapping population of 242 F$_{2}$ individuals being employed. The map was used to select 11 markers that were investigated for polymorphisms between the restorer donor line and 46 recipient lines. A set of four RAPD markers, one in coupling phase with the restorer allele and three with the non-restorer allele, which were informative in all 46 combinations, were used in marker assisted selection of plants homozygous for the restorer allele. A total of 906 homozygous restored plants were found among the 4605 BC$_{1}$F$_{2}$ plants analysed. Phenotypic data of a subset of the classified plants was compared with the RAPD data and the expected number of recombinants was calculated from the map data. A close correspondence between the expected and observed numbers of plants with a deviating phenotype was found. Thus, use of a set of dominant RAPD markers provides a way obtaining reliable data for marker-assisted selection.
Starting Page	449
Ending Page	456
Page Count	8
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	3
Issue Number	6
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Improvement of the recessive genic male sterile lines with a subgenomic background in Brassica napus by molecular marker-assisted selection

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.

Introgression of Brassica rapa subsp. sylvestris blackleg resistance into B. napus

Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus)

Genome mapping, molecular markers and marker-assisted selection in crop plants

Molecular Tagging and Selection for Sugar Type in Carrot Roots Using Co-dominant, PCR-based Markers

Development of INDELs markers in oilseed rape (Brassica napus L.) using re-sequencing data

Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.

Marker-assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers

Similar Documents

Improvement of the recessive genic male sterile lines with a subgenomic background in Brassica napus by molecular marker-assisted selection

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.

Introgression of Brassica rapa subsp. sylvestris blackleg resistance into B. napus

Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus)

Genome mapping, molecular markers and marker-assisted selection in crop plants

Molecular Tagging and Selection for Sugar Type in Carrot Roots Using Co-dominant, PCR-based Markers

Development of INDELs markers in oilseed rape (Brassica napus L.) using re-sequencing data

Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.

Marker-assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers