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| Content Provider | Springer Nature Link |
|---|---|
| Author | Nebauer, Sergio G. Arrillaga, Isabel del Castillo Agudo, Lucas Segura, Juan |
| Copyright Year | 2000 |
| Abstract | Transgenic plants of the aromatic shrub Lavandula latifolia (Lamiaceae) were produced using Agrobacterium tumefaciens-mediated gene transfer. Leaf and hypocotyl explants from 35–40-day old lavender seedlings were inoculated with the EHA105 strain carrying the nptII gene, as selectable marker, and the reporter gusA gene with an intron. Some of the factors influencing T-DNA transfer to L. latifolia explants were assessed. Optimal transformation rates (6.0 ± 1.6% in three different experiments) were obtained when leaf explants precultured for 1 day on regeneration medium were subcultured on selection medium after a 24 h co-cultivation with Agrobacterium. Evidence for stable integration was obtained by GUS assay, PCR and Southern hybridisation. More than 250 transgenic plants were obtained from 37 independent transformation events. Twenty-four transgenic plants from 7 of those events were successfully established in soil. β-glucuronidase activity and kanamycin resistance assays in greenhouse-grown plants from two independent transgenic lines confirmed the stable expression of both gusA and nptII genes two years after the initial transformation. Evidence from PCR data, GUS assays and regeneration in the presence of kanamycin demonstrated a 1:15 Mendelian segregation of both transgenes among seedlings of the T1 progeny of two plants from one transgenic L. latifolia line. |
| Starting Page | 539 |
| Ending Page | 552 |
| Page Count | 14 |
| File Format | |
| ISSN | 13803743 |
| Journal | Molecular Breeding |
| Volume Number | 6 |
| Issue Number | 6 |
| e-ISSN | 15729788 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2000-01-01 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science |
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