NDLI: TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops

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Molecular Breeding : Volume 37, Issue 1, January 2017

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TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops

Content Provider	Springer Nature Link
Author	Kumar, Anishkumar P. K. McKeown, Peter C. Boualem, Adnane Ryder, Peter Brychkova, Galina Bendahmane, Abdelhafid Sarkar, Abhimanyu Chatterjee, Manash Spillane, Charles
Copyright Year	2017
Abstract	TILLING (Targeting Induced Local Lesions in Genomes) by Sequencing (TbyS) refers to the application of high-throughput sequencing technologies to mutagenised TILLING populations as a tool for functional genomics. TbyS can be used to identify and characterise induced variation in genes (controlling traits of interest) within large mutant populations, and is a powerful approach for the study and harnessing of genetic variation in crop breeding programmes. The extension of existing TILLING platforms by TbyS will accelerate crop functional genomics studies, in concert with the rapid increase in genome editing capabilities and the number and quality of sequenced crop plant genomes. In this mini-review, we provide an overview of the growth of TbyS and its potential applications to crop molecular breeding.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	37
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-01-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TILLING by Sequencing Induced variation TbyS Mutagenesis CRISPR/Cas9 Genome editing Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops

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