NDLI: Development of transgenic rice plants expressing maize anthocyanin genes and increased blast resistance

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Development of transgenic rice plants expressing maize anthocyanin genes and increased blast resistance

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Development of transgenic rice plants expressing maize anthocyanin genes and increased blast resistance

Content Provider	Springer Nature Link
Author	Gandikota, Madhuri de Kochko, Alexandre Chen, Lili Ithal, Nagabhushana Fauquet, Claude Reddy, Arjula R.
Copyright Year	2001
Abstract	The functional association of flavonoids with plant stress responses, though widely reported in the literature, remains to be documented in rice. Towards this end we chose a transgenic approach with well characterized regulatory and structural genes from maize involved in flavonoid biosynthesis. Activation of anthocyanin pathway in rice was investigated with the maize genes. Production of purple anthocyanin pigments were observed in transformed Tp309 (a japonica rice variety) calluses upon the introduction of the maize regulatory genes C1 (coloured-1), R (red) and the structural gene C2 (coloured-2, encoding chalcone synthase). In addition, stable transgenic plants carrying the maize C2 gene under the control of the maize Ubiquitin promoter were generated. A localized appearance of purple/red pigment in the leaf blade and leaf sheath of R$_{0}$ C2 transgenic seedlings was observed. Such a patchy pattern of the transgene expression appears to be conditioned by the genetic background of Tp309, which is homozygous for dominant color inhibitor gene(s) whose presence was unravelled by appropriate genetic crosses. Southern blot analysis of the transgenic plants demonstrated that c2 cDNA was integrated into the genome. Western blot analysis of these primary transgenics revealed the CHS protein while it was not detected in the control untransformed Tp3O9, suggesting that Tp309 might have a mutation at the corresponding C2 locus or that the expression of this gene is suppressed in Tp309. Further analysis of C2 transgenics revealed CHS protein only in three out of sixteen plants that were western-positive in the R$_{0}$ generation, suggesting gene silencing. Preliminary screening of these R$_{1}$ plants against the rice blast fungus Magnaporthe grisea revealed an increase in resistance.
Starting Page	73
Ending Page	83
Page Count	11
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	7
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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