NDLI: Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing

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Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing

Content Provider	Springer Nature Link
Author	Missiou, Anastasia Kalantidis, Kriton Boutla, Alexandra Tzortzakaki, Sergia Tabler, Martin Tsagris, Mina
Copyright Year	2004
Abstract	In this study we applied RNA silencing to engineer potato plants that are resistant to potato virus Y (PVY). We expressed double-stranded (ds) RNA derived from the 3′ terminal part of the coat protein gene of PVY, which is highly conserved in sequence amongst different PVY isolates, in transgenic potatoes of the commercial variety ‘Spunta’. Transgenic plants were analyzed for generation of transgene-derived short interfering RNAs (siRNAs) prior to virus inoculation. Twelve of fifteen transgenic lines produced siRNAs and were highly resistant to three strains of PVY, each belonging to three different subtypes of the virus (PVY$^{N}$, PVY$^{O}$ and PVY$^{NTN}$). Infection of transgenic plants with Potato virus X (PVX) simultaneously or prior to the challenge with PVY did not interfere with PVY-resistance.
Starting Page	185
Ending Page	197
Page Count	13
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	14
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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