NDLI: Single-nucleotide polymorphism identification and genotyping in Camelina sativa

Content Provider	Springer Nature Link
Author	Singh, Ravinder Bollina, Venkatesh Higgins, Erin E. Clarke, Wayne E. Eynck, Christina Sidebottom, Christine Gugel, Richard Swdon, Rod Parkin, Isobel A. P.
Copyright Year	2015
Abstract	Camelina sativa, a largely relict crop, has recently returned to interest due to its potential as an industrial oilseed. Molecular markers are key tools that will allow C. sativa to benefit from modern breeding approaches. Two complementary methodologies, capture of 3′ cDNA tags and genomic reduced-representation libraries, both of which exploited second generation sequencing platforms, were used to develop a low density (768) Illumina GoldenGate single nucleotide polymorphism (SNP) array. The array allowed 533 SNP loci to be genetically mapped in a recombinant inbred population of C. sativa. Alignment of the SNP loci to the C. sativa genome identified the underlying sequenced regions that would delimit potential candidate genes in any mapping project. In addition, the SNP array was used to assess genetic variation among a collection of 175 accessions of C. sativa, identifying two sub-populations, yet low overall gene diversity. The SNP loci will provide useful tools for future crop improvement of C. sativa.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	35
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-01-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Camelina sativa Reduced representation SNP Genetic mapping Diversity Polyploidy Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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